China Journal of Chinese Materia Medica, the 1st in the field of TCM, is supervised by China Association for Science and Technology and sponsored by Institute of Chinese Pharmaceutical Association. The journal is China's earliest comprehensive core journal of traditional Chinese medicine, and always maintains the circulation top in the professional areas. The journal publishes the latest research and progress of traditional Chinese medicine and takes a leading position in numbers of articles published, downloads and citations among all journals in this discipline.
Its scope covers new achievements, technologies, methods, experiences and concepts resulting from the research on Chinese materia medica pursuant to Chinese medical and pharmaceutical theories, traditional experiences, and modern science and technology, including medicinal resources and identification, cultivation, processing, preparation, chemistry, pharmacology, theory of Chinese pharmacy and clinical practice, bencaological study.
The journal is included in CA, JST and CSCD.
Honorary Editor-in-Chief Xiao Peigen Editor-in-Chief Wang Yongyan
Associate Editors Zhang Boli, Hu Zhibi, Yao Xinsheng, Li Lianda, Li Dapeng, Yang Baofeng, Zhou Chaofan, Huang Luqi, Chen Shilin, Li He.
Executive Editorial Board Cai Shaoqing, Chen Shilin
To identify origin of the medicinal materials Dryopteridis Crassirhizomatis Rhizoma by using the psbA-trnH sequence, the polymerase chain reaction ( PCR) amplification and product sequencing of the experimental samples were performed. In order to expand the scope of the study, the psbA-trnH sequences of 8 genera and 3 species were downloaded from Gen Bank for analysis. DNAMAN 8.0 software was used to show splicing and comparison results of the peak diagrams with analysis of them, and MEGA 6.0 software was to calculate K2P genetic distances and establish clustering tree adjacent genus. The results showed that by using the psbA-trnH sequence, Dryopteridis Crassirhizomatis Rhizoma, its original plant and other easy-confused medicinal materials and plants can be distinguished with each other obviously, with the psbA-trnH sequence of Dryopteridis Crassirhizomatis Rhizoma completely consistent with that of its original plant. Consequently, it is revealed that it's feasible to identify Dryopteridis Crassirhizomatis Rhizoma and its original plant, and separate from its adulterants by means of the psbA-trnH sequence, which can provide more scientific bases for the further study of the identification of the ferny medicinal herbs.
This paper was aimed to investigate the protective effects of luteolin (Lut) against acetaminophen (APAP)-induced damage in L02 liver cells. CCK-8 was used to detect the cell activation of L02 cells treated by different Lut. The concentration and time of APAP induced L02 cell damage was screened. The effect of Lut on APAP induced apoptosis of L02 cells was detected by cell morphological observation, CCK-8 assay and flow cytometry. The contents of MDA, GSH and SOD activity in cell supernatant were detected by colorimetric assay. The expression of apoptosis-related genes Bax, Bcl-2 and caspase-3 was detected by RT-PCR. The results showed that Lut in 2.5–40 μmol/L range does not affect the activity of L02 cells; 12 mmol/L APAP acts on L02 cell 12 h to establish liver cell injured model. Compared with the model group, the cell status of Lut group was significantly improved, the cell body was increased, the adherence ability was recovered, and the apoptosis rate was obviously decreased. MDA content decreased significantly (P < 0.05, P < 0.01), GSH and SOD activity significantly increased (P < 0.05, P < 0.01), at the same time, it could up-regulate expression of Bcl-2 m RNA and down-regulate the expression of Bax and caspase-3 m RNA. In conclusion, Lut has protective effect on APAP induced L02 cell injury, and its mechanism may be related to the reduction of oxidative stress and inhibition of apoptosis.
A new alkaloid was isolated from the leaves of Macleaya cordata with 95% ethanol extraction and isolation was conducted with column chromatography and preparation HPLC. The new structure was elucidated as 6'-hydroxy-2', 3'-dimethoxyarnottianamide based on spectroscopic date.
The open reading frame (ORF) of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (HDR) was cloned from Phlegmariurus carinatus by RT-PCR method and the sequence was analyzed by bioinformatics tools. After searching the transcriptome dataset of P. carinatus, one unique sequence encoding HDR was discovered. The primers were designed according to the cDNA sequence of HDR from the dataset. And then, the ORF of HDR, named as PcHDR1 (GenBank Accession number: JQ957845), was cloned by RT-PCR strategy with the template of mixed RNA extracted from roots, stem and leaf of P. carinatus. The bioinformatic analysis of this gene and its corresponding protein was performed. The ORF of PcHDR1 consisted of 1 437 base pairs (bp), encoding one polypeptide with 478 amino acids. The sequence comparison showed that PcHDR1 is closest with GbHDR (Ginkgo biloba), and the sequence homology was up to 78%. Bioinformatics prediction and analysis indicated that PcHDR1 protein contained a conserved domain of LytB, without transmembrane region and signal peptides. This study cloned and analyzed HDR from P. carinatus. The result will provide a foundation for exploring the function of PcHDR1 involved in terpene biosynthesis in P. carinatus plants.
This study aimed to provide guidance for the heterogenous gene expression, gene prediction and species evolution by analyzing codon usage bias of Catharanthus roseus. The codon composition and usage bias of 30,437 high-confidence coding sequences from C. roseus were analyzed and the proportions of rare codons of Escherichia coli and Saccharomyces cerevisiae in 25 genes involved in the biosynthesis of terpenoid indole alkaloids (TIAs) in C. roseus were calculated. The results showed that the average GC content of the genes was 42.47%; the average GC content of the third bases in codon was 35.89%. The relative synonymous codon usage ( RSCU) of 28 codons were greater than 1 and 26 of them ended with A or T. The above 25 genes involved in TIA biosynthesis contained much more rare condons of E. coli than that of S. cerevisiae. It was concluded that C. roseus mainly prefered the codons ending with A or T and the rule of codon usage was more different to E. coli than S. cerevisiae. Thus, S. cerevisiae may be the more suitable host for heterologous expression of these genes.
This paper was aimed to establish a method for coronary heart disease (CHD) in rats with Qi-deficiency and blood stasis by comparing different model establishment methods (L group: ligation of coronary artery, EL group: combination of exercise fatigue and ligation of coronary artery, DL group: combination of diet and ligation of coronary artery, DEL group: combination of diet, exercise fatigue and ligation of coronary artery). After 6 weeks post-operation, the method of L (ligation of coronary artery) and DL, EL, DEL (multi-originated information complex) were used to successfully establish CHD model of qi-deficiency and blood-stasis syndrome type in rats. Model set up by using the compound factors (DL, EL, DEL) and that through simple ligation of coronary artery (L) were used to compare the clinical etiology and the link. DL, EL and DEL were more closely with relevant theoretical system of traditional Chinese medicine (TCM) and have a certain advantage in completely reflect the characteristics of TCM syndrome. Among three kinds of compound factors models, EL model was more consistent with clinical practical reasons and characteristics of the disease than DL and DEL models. Through EL the CHD deficiency of blood stasis rat model of combined disease could be controlled and good repeated.
Using the latest 454 GS FLX platform and Titanium regent, a substantial expressed sequence tags (ESTs) dataset of
Ephedra sinica was produced, and the profile of gene expression and function gene of which were investigated. A total of 48 389 reads with an average length of 373 bp were generated. These 454 reads were assembled into 18 801 unigenes, which were all 454 sequencing identified. A total number of 10 531 unigenes (56.0%) were annotated using BLAST searches ( E-value ≤ 1 × 10
−5) against the Nr, Nt, TAIR, Swiss Prot and KEGG databases. With respect to genes related to ephedrine biosynthesis, 19 unigenes (encoding 9 enzymes) were found. A total of 97 putative genes encoding cytochrome P450s were also discovered. Data presented in this study will provide an important resource for the scientific community that is interested in the functional genomics and secondary metabolism of
E. sinica.
