China Journal of Chinese Materia Medica, the 1st in the field of TCM, is supervised by China Association for Science and Technology and sponsored by Institute of Chinese Pharmaceutical Association. The journal is China's earliest comprehensive core journal of traditional Chinese medicine, and always maintains the circulation top in the professional areas. The journal publishes the latest research and progress of traditional Chinese medicine and takes a leading position in numbers of articles published, downloads and citations among all journals in this discipline.
Its scope covers new achievements, technologies, methods, experiences and concepts resulting from the research on Chinese materia medica pursuant to Chinese medical and pharmaceutical theories, traditional experiences, and modern science and technology, including medicinal resources and identification, cultivation, processing, preparation, chemistry, pharmacology, theory of Chinese pharmacy and clinical practice, bencaological study.
The journal is included in CA, JST and CSCD.
Honorary Editor-in-Chief Xiao Peigen Editor-in-Chief Wang Yongyan
Associate Editors Zhang Boli, Hu Zhibi, Yao Xinsheng, Li Lianda, Li Dapeng, Yang Baofeng, Zhou Chaofan, Huang Luqi, Chen Shilin, Li He.
Executive Editorial Board Cai Shaoqing, Chen Shilin
The chemical constituents of the water extraction of the aerial parts of Isodon excisoides were investigated by various chromatographic methods including D-101 macroporous adsorptive resins, silica gel, Sephadex LH-20, MCI and semi-preparative HPLC. As a result, six compounds were separated and purified. By analyses of the HR-ESI-MS, 1 D and 2 D NMR spectra, their structures were determined as 3-O-β-D-allopyranosyl-1-octen-3-ol (1), blumenol A (2), lumichrome (3), loliolide (4), cirsiliol (5) and pedalitin (6). Compound 1 was a new compound, and compounds 2–4 were isolated from this plant for the first time.
Volatile oils are important active components in traditional Chinese medicine, but their components are complicated and unstable. It is common to use cyclodextrin inclusion technique to improve the stability of volatile oils and make them easier to be prepared. At present, β-cyclodextrin (β-CD) is the most common inclusion material. The evaluation indicators for inclusion technique usually contain the inclusion rate and the oil content in the inclusion compound. However, the articles about the study on selecting inclusion materials for volatile oils were few. In this paper, menthol, the main active ingredient of mint volatile oil, was used as model drug, while β-CD and hydroxypropyl-β-cyclodextrin (HP-β-CD) were used as the inclusion materials. Inclusion equilibrium constant (K), solubilization ratio were investigated, and the results were combined with IR, DSC and TG to verify the formation of inclusion complexes. It turned out that in the range of 0–15 mmol·L−1, the solubility of menthol was increased linearly with the increase of HP-β-CD concentration, with AL-type phase solubility diagram, K = 3 188.62 L·mol−1; in the range of 0–12.5 mmol·L−1, the solubility of menthol was increased linearly with the increase of β-CD concentration, K = 818.73 L·mol−1. When the concentration was over 12.5 mmol·L−1, the solubility of menthol appeared to be a negative deviation with the increase of β-CD concentration, with AN-type solubility diagram. The above results showed that the inclusion behavior was different between β-CD and HP-β-CD, laying a foundation for further study on inclusion complexes of volatile oil.
Organic acids are widely distributed in plants and related products, and participate in a wide range of metabolic pathways (e.g., tricarboxylic acid cycle), showing diverse pharmacological activities. As a widely used Chinese patent medicine, its adverse reactions are often reported. Therefore, we should further clarify the chemical components of Shenfu injection, and prepare strict quality standards to ensure the safety and effectiveness of its clinical use. Shenfu injection is prepared from red ginseng (steamed roots of Panax ginseng) and black prepared lateral roots of Aconitum carmichaelii (Heishunpian) by using modern extraction process, and organic acids are regarded as one of its main components. In current study, a hydrophilic interaction chromatography (HILIC) coupled with mass spectrometric method (HILIC-LC-MS) was developed and validated for the simultaneous determination of 14 organic acids, including cinnamic acid, ferulic acid, 4-hydroxylbenzoic acid, L-(+)-lactic acid, adipic acid, fumaric acid, caffeic acid, succinic acid, maleic acid, malonic acid, D-malic acid, (−)-shikimic acid, D-tartaric acid, and quinic acid in Shenfu injection. Satisfactory retention and separation were achieved for all organic acids on HILIC chromatographic column. Except cinnamic acid (231 μg·L−1), lactic acid (113 μg·L−1) and malonic acid (32.5 μg·L−1), the limit of quantitation for the remaining 11 compounds were less than 10 μg·L−1. D-Malic acid, malonic acid, quinic acid, L-(+)-lactic acid, and cinnamic acid were observed to have higher contents in Shenfu injection (>1.89 mg·L−1), whereas caffeic acid and adipic acid were undetectable in all batches. Above all, the developed method is suitable for the simultaneous determination of organic acids in Shenfu and some other traditional Chinese medicine injections.
To investigate the effects and mechanisms of total flavones of Epimedium (TFE) on oxidative stress induced by myocardial ischemia/reperfusion injury in rats, forty male SD rats were randomly divided into sham operated group, model group, diltiazem group and flavonoids of Epimedium low and high doses groups, with 8 rats in each. Myocardial ischemia/reperfusion injury model was induced by ligaturing the left anterior descending artery for 30 min followed reperfusion for 4 h after TFE was taken by intragastric administration for 4 days. The degree of myocardial infarct was observed by N-BT staining. The concentrations of MDA and activities of SOD and T-AOC in cardiac tissue were measured by colorimetry. Serum TnI concentrations were checked by ELISA. HE stain was used to observe myocardium structure under light microscope. The expression levels of SIRT1 and Nrf2 in cardiac tissue were evaluated by immunohistochemistry method and Western blot, respectively. Compared with the model group, the degree of myocardial infarct, the MDA concentration in cardiac tissue and the levels of TnI in serum significantly decreased in the diltiazem group and flavonoids of Epimedium low and high doses groups (P < 0.05 or P < 0.01). The flavonoids of Epimedium low and high doses groups and the diltiazem group also showed improvements in myocardium structure under ischemia/reperfusion injury. TFE significantly increased the activity of SOD and T-AOC and the expression of SIRT1 and Nrf2 in cardiac tissue when compared with the model group (P < 0.05 or P < 0.01). Therefore, TFE can increase anti-peroxidant capacity of myocardium tissue by using intrinsically anti-oxidant signaling pathway of SIRT1 and Nrf2, which can inhibit irreversible damage of cardiomyocytes in myocardial ischemia/reperfusion injury and protect normal function of cardiac tissue.
Using reversed-phase high performance liquid chromatography, nine ginsenosides were simultaneously separated on an Ultimate
® C
18 column with high-resolution and high purity of each chromatographic peak. Adopting the QAMS quality evaluation model for traditional Chinese medicines, ginsenoside Rb
1 was used as the internal reference substance, and the relative correction factors (RCFs) and the relative retention values (RT
Rs) of ginsenosides Rg
1, Re, Rf, Rb
1, Rc, Rb
2, Rb
3, Rd and 20(
S)-ginsenoside Rg
3 to ginsenoside Rb
1 were calculated individually. Through a series of methodology evaluations, and positioned by the red ginseng reference chromatograph and RTVs, nine ginsenosides in red ginseng were simultaneously assayed only by quantitatively determined ginsenoside Rb
1.
Based on the ecological suitability analysis of producing area of
Panax ginseng and the selection criteria of farmland cultivation in the global range, we aimed to provide scientific basis for rational planning, production layout and standardized planting in the farmland. We analyzed the data based on the ecological factors from 271 sample plots of
Panax ginseng, including both the traditional producing regions recorded in past dynasties medicinal works and the popular production regions in the global scale, using global geographic information system for medicinal plant (GMPGIS) developed by Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences. We concluded that the suitable producing areas in global for
Panax ginseng mainly included the United States, Canada, China, Russia, Japan, North Korea, France, Italy, Ukraine, and South Korea. In addition, the suitable producing areas in China mainly included Heilongjiang, Jilin, Liaoning, Shanxi, Gansu, Hubei, Sichuan, Inner Mongolia, Shandong, and Shanxi. Besides, based on the references and the experience of
Panax ginseng-producing and our many years’ work on the 1 000-hectare plantation of
Panax ginseng, we established a standard land selection protocol for cultivation of
Panax ginseng. The use of GMPGIS to select the most optimum
Panax ginseng production regions provides a new scientific basis for introduction, cultivation, tending, protection, cultivation normalization for
Panax ginseng and the standard land selection protocol would lay a solid foundation for the high-quality
Panax ginseng production.
