Sponsor(s): Institute of Chinese Pharmaceutical Association
24 issues per year
Current Issue: Issue 01, 2014
Journal official website:http://zgzye.cbpt.cnki.net/WKE/WebPublication/index.aspx?mid=zgzye
China Journal of Chinese Materia Medica, the 1st in the field of TCM, is supervised by China Association for Science and Technology and sponsored by Institute of Chinese Pharmaceutical Association. The journal is China's earliest comprehensive core journal of traditional Chinese medicine, and always maintains the circulation top in the professional areas. The journal publishes the latest research and progress of traditional Chinese medicine and takes a leading position in numbers of articles published, downloads and citations among all journals in this discipline. Its scope covers new achievements, technologies, methods, experiences and concepts resulting from the research on Chinese materia medica pursuant to Chinese medical and pharmaceutical theories, traditional experiences, and modern science and technology, including medicinal resources and identification, cultivation, processing, preparation, chemistry, pharmacology, theory of Chinese pharmacy and clinical practice, bencaological study. The journal is included in CA, JST and CSCD.
Zhang Boli, Hu Zhibi, Yao Xinsheng, Li Lianda, Li Dapeng, Yang Baofeng, Zhou Chaofan, Huang Luqi, Chen Shilin, Li He.
Executive Editorial Board
Cai Shaoqing, Chen Shilin
China Journal of Chinese Materia Medica,2014,Vol 39,No. 01
Objective: To observe the effect of pure total flavonoids from Citrus (PTFC) on the hepatic fatty degen-eration，inflammation，oxidative stress and SIRT1/PGC-1α expressions in mice with non-alcohol steatohepatitis (NASH), and discuss the action mechanism of PTFC on NASH. Method: Mice were given high-fat diet for 16 weeks to induce the NASH model. Since the seventh week after the model establishment，the mice were intervened with 100, 50 and 25 mg·kg -1·d -1 PTFC for 10 weeks. The pathologic changes in hepatic tissues were observed with HE staining. The contents of TG，CHOL in hepatic tissue，as well as the levels of AST，ALT in serum were detected by using the biochemical process. The expression of SIRT1，PGC-1α and MnSOD mRNA in hepatic tissues were detected with Real-time PCR assay. SIRT1，PGC-1α protein and 8-OHdG expressions were determined with the immunohistochemical method. The SOD level in hepatic tissues was tested by the xanthine oxidase method. The MDA content in hepatic tissues was examined by the thiobarbituric acid method. Result: The contents of TG，CHOL，NAFLD activity scores and ALT level in serum in hepatic tissues of mice in the model induced by fat-rich diet were remarkably higher than that of the control group (P < 0.01). The SIRT1，PGC-1α，MnSOD mRNA and protein expression in hepatic tissues were significantly lower than that of the control group (P < 0.01). The expres-sion of 8-OHdG and the content of MDA in hepatic tissues were significantly higher than that of the control group (P < 0.01). After the intervention with different doses of PTFC，the NAFLD activity scores，the content of TG and the level of AST in serum were notably lower than that of the control group (P < 0.01，P < 0.05); whereas the SIRT1，PGC-1α，MnSOD mRNA and protein expression were remarkably higher than that of the control group (P < 0.01，P < 0.05)，with the significant decrease in the expression of 8-OHdG and the content of MDA (P < 0.01). Conclusion: Oxidative stress/lipid peroxidation enhancement in NASH mice induced by high-fat diet may be relat-ed to the changes in SIRT1/PGC-1α signal transduction pathway. PTFC could enhance the anti-oxidant capacity in liver，relieve the damage of reactive oxygen during the fatty acid metabolic process，and prevent NASH from the occurrence and development by regulating the SIRT1/PGC-1α signal pathway.
Effect of combined administration of Angelica polysaccharide and cytarabine on liver of human leukemia NOD/SCID mouse model
China Journal of Chinese Materia Medica,2014,Vol 39,No. 01
Leukemia is a type of malignant tumors of hematopoietic system with the abnormal increased immature leukemia cells showing metastasis and invasion ability. Liver is one of the main targets of the leukemia cells spread to, where they may continue to proliferate and differentiate and cause liver function damage,even liver failure. Our previous studies showed that Angelica polysscharides (APS), the main effective components in Angelica sinensis of Chinese traditional medicine, was able to inhibit the proliferation and induced differentiation of the leukemia cells, however, its effect on the liver during the treatment remains elucidated. In the present study, the human leukemia NOD/SCID mouse model were established by implantation human leukemia K562 cells line, then the leukemia mouse were treated with APS, Ara-c or APS + Ara-c respectively by peritoneal injection for 14 days, to explore the effect and mechanism of the chemicals on the mouse liver. Compared to the human leukemia NOD/SCID mouse model group with the treatments of APS,Ara-c and APS + Ara-c, we found that severe liver damage and pathological changes of the liver were able to alleviate: First, the number of white blood cells in the peripheral blood was significantly lower and with less transplanted K562 leukemia cells; Second, liver function damage was alleviated as liver function tests showed that alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total bilirubin (TBiL) were significantly reduced, while the albumin (Alb) was notably increased; Third, liver antioxidant ability was improved as the activities of the antioxidant enzymes glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) were significantly increased, and the contents of GSH and malonaldehyde (MDA) were decreased significantly in the liver; Fourth, the inflammation of the liver was relieved as the level of IL-1 β and IL-6, the inflammatory cytokines,were decreased significantly in the liver. Fifth, liver index was increased as the pathological observation showed that leukemia cells with diffused infiltration into the liver lobules were significantly reduced and with a remarkable increase of apoptotic positive cell rate by TUNEL test. Furthermore, the APS + Ara-c combined administration showed an even more significant positive effect. In conclusion, the APS, Ara-c therapy reduced the accumulation of leukemia cells within the liver, reduced the liver function damage and levels of inflammatory factors, improved antioxidant capacity of the liver tissue and thus alleviate the pathological changes of the liver. Moreover, the APS + Ara-c combination therapy may have an additive effect.