Sponsor(s):Tianjin Institute of Pharmaceutical Research；Chinese Pharmaceutical Association
24 issues per year
Current Issue: Issue 24, 2020
Chinese Traditional and Herbal Drugs is supervised by Chinese Pharmaceutical Association and sponsored by Tianjin Institute of Pharmaceutical Research and Chinese Pharmaceutical Association. Launched in 1970, the journal is an academic journal with a broad scope in publishing research papers, brief reports, reviews, dissertations, and special treatises on the recent achievements of basic study, production, quality control, and clinic application on traditional Chinese medicine and Chinese materia medica. The journal is included in CA, JST and CSCD.
Mechanism of wangzaozin A, an ent-kaurane diterpene, on cytoskeletal rearrangement and migration inhibition of A549 cells
Chinese Traditional and Herbal Drugs,2020,Vol 51,No. 24
Objective To investigate the mechanism of cytoskeletal recombination and migration inhibition induced by wangzaozin A, an ent-kaurane diterpene, in A549 cells. Methods The effects of wangzaozin A on cytotoxicity, cell morphology, cytoskeleton, and protein expression as well as cell migration were detected in A549 cells by using MTT, microscope observation, Western blotting, immunofluorescence assay and scratch assay. Results Wangzaozin A induced significant changes in cell morphology at 24, 48, and 72 h, including increased pseudopods, stretched pseudopods, and flattened nucleus in A549 cells. Moreover, microtubules and keratin fibers networks in A549 cells also showed obvious rearrangement, which indicated the cytoskeleton had gone through a continuous recombination process. Further, wangzaozin A significantly increased the phosphorylation of extracellular regulated protein kinase (ERK), microtubule-associated protein 4 (MAP4), keratin 8 (K8) ( P < 0.05, 0.01), while wangzaozin A-induced phosphorylation of MAP4 and K8 were suppressed in A549 cells treated with ERK inhibitors U0126 ( P < 0.05, 0.01); wangzaozin A inhibited the migration of A549 cells in a concentration- and time-dependent manner. Conclusion Wangzaozin A can upregulate the phosphorylation of MAP4 and K8 by activating ERK signaling pathway, which can significantly increase the dynamics of MTs and KFs, disturb the dynamic balance of the cytoskeleton, and inhibit the migration of A549 cells.