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Brief

Chinese Traditional and Herbal Drugs is supervised by Chinese Pharmaceutical Association and sponsored by Tianjin Institute of Pharmaceutical Research and Chinese Pharmaceutical Association. Launched in 1970, the journal is an academic journal with a broad scope in publishing research papers, brief reports, reviews, dissertations, and special treatises on the recent achievements of basic study, production, quality control, and clinic application on traditional Chinese medicine and Chinese materia medica. The journal is included in CA, JST and CSCD.
Editorial Board

Editor-in-Chief
Tang Lida

Director
Chen Changqing

Neuroprotective effect of borneol combined with astragaloside Ⅳ and Panax notoginseng saponins in cerebral ischemia reperfusion injury rat model through Notch signaling pathway

OUYANG Bo;LIU Xiao-dan;YANG Xiao-qian;DING Huang;HUANG Xiao-ping;DENG Chang-qing

Chinese Traditional and Herbal Drugs,2020,Vol 51,No. 23

Objective To observe the neuroprotective effect of borneol combined with astragaloside Ⅳ (AST Ⅳ) and Panax notoginseng saponins (PNS) on rat models with cerebral ischemia reperfusion injury (CIRI) from the perspective of the Notch signaling pathway. Methods SD rats were randomly divided into the sham operation group, model group, borneol (7.5 mg/kg) group, AST Ⅳ (25 mg/kg) group, PNS (10 mg/kg) group, AST Ⅳ (10 mg/kg) + PNS (25 mg/kg) group, low-dose borneol (7.5 mg/kg) + AST Ⅳ (25 mg/kg) + PNS (10 mg/kg) group, high-dose borneol (15 mg/kg) + AST Ⅳ (20 mg/kg) + PNS (50 mg/kg) group and edaravone (4 mg/kg) group. Rats in the sham operation and model groups received intragastric administration of 0.5% CMC-Na (10 mL/kg); those in the edaravone group received edaravone (2.5 mL); the ones in the remaining administration groups were treated with 2.5 mL of the corresponding drugs by gavage. The medication was provided twice per day at a 12-h interval. The right middle cerebral artery of the rat was occluded by a suture 2 h after the last administration to establish the CIRI model. After 2 h of ischemia and 22 h of reperfusion, the neurological deficit scoring was conducted and pathological changes in the ischemic cortex of rats were observed after HE staining. The protein expression levels of neuron-specific nuclear (NeuN) and endothelial barrier antigen (EBA) in the ischemic cortex were detected by immunohistochemistry. The protein expression levels of vascular endothelial growth factor (VEGF), Notch1, and the intracellular domain of Notch (NICD) in the ischemic cortex were detected by Western blotting. Results The neurological deficit score and cell damage rate in the model group were significantly increased ( P < 0.01); the neurological deficit score and cell damage rate in each administration group were significantly reduced ( P < 0.05, 0.01), and the effect of borneol + AST Ⅳ + PNS was better than that of any single drug or AST Ⅳ + PNS ( P < 0.05, 0.01). NeuN and EBA protein expression levels were significantly decreased in the ischemic cortex of the model group ( P < 0.01), while those in each administration group were significantly enhanced ( P < 0.05, 0.01). The effect of borneol + AST Ⅳ + PNS was better than that of any single drug or AST Ⅳ + PNS ( P < 0.05, 0.01). In the model group, VEGF protein expression was increased significantly ( P < 0.05), while NICD and Notch1 protein expression levels had no significant change. The protein expression levels of VEGF, NICD, and Notch1 were significantly up-regulated in the borneol + AST Ⅳ + PNS group ( P < 0.01), and the effect of the combination of three drugs was better than that of any single drug or AST Ⅳ + PNS ( P < 0.05, 0.01). Conclusion Borneol, AST Ⅳ, and PNS have the effects of preventing neuronal and cerebral microvascular damage after CIRI, and the protective effect of their combination against cerebral ischemic injury was better than that of any single drug or AST Ⅳ + PNS, which might be related to the activation of the Notch signaling pathway and the up-regulation of VEGF expression.

Mechanism of Shaoyao Decoction in treatment of ulcerative colitis based on network pharmacology and molecular docking technology

LU Ai-ni;WANG De-long;ZHAO Fang;CHEN Rui-jie;CHEN Wei-qiao;ZHENG Hong-bin;JI Xu-ming

Chinese Traditional and Herbal Drugs,2020,Vol 51,No. 23

Objective Network pharmacology and molecular docking technology were used to study the material basis and possible mechanism of Shaoyao Decoction in the treatment of ulcerative colitis (UC). Methods TCMSP and TCMID were used to obtain the potential active components and targets of Shaoyao Decoction. GeneCard and OMIM were used to search disease targets. The common targets obtained by matching drug targets with disease targets were imported into String to construct a PPI network, and Cyto NCA plug-in was used to screen key targets. The network diagram was plotted by connecting the key targets with the corresponding components, so as to screen the key components. GO and KEGG enrichment analyses were performed on key targets. SYBYL-X2.0 software was used to dock the molecules of the key components with the key targets. The rat UC model was replicated in vivo. After the intervention with Shaoyao Decoction, the disease activity index (DAI) was observed; the colonic pathological damage was evaluated; the levels of TNF-α, IL-4, and CXCR4 were detected by ELISA. Results A total of 424 potential active components were found in Shaoyao Decoction. The key components included quercetin, palmitic acid, catechin, and procyanidins, etc. Its 41 key targets for UC were mainly related to the positive regulation of transcription, the negative regulation of apoptosis process, signal transduction, and other biological processes. The key targets played a role in treating UC through signaling pathways such as TNF, HIF-1, cancer pathway, TLR, PI3 K-Akt, et al. Molecular docking results showed that key components had good binding activities with corresponding targets. Shaoyao Decoction improved colonic pathological damage, down-regulated the levels of TNF-α and CXCR4, and up-regulated the level of IL-4 in vivo. Conclusion Shaoyao Decoction exerts the therapeutic effects against UC via “multiple components, multiple targets, and multiple pathways”, which has laid a foundation for further study of its mechanism.