Sponsor(s):Xinjiang Academy of Agricultural Sciences, Xinjiang Agricultural University, Agricultural Society of Xinjiang Uygur Autonomous Region
12 issues per year
Current Issue: Issue 04, 2020
Xinjiang Agricultural Sciences aims to serve the agriculture and promote agricultural production in the arid areas, with highlights on the scientific and technological achievements and advanced technologies for agriculture in the arid areas.
Editor-in-Chief: Cheng Zhili
Cloning and Molecular Characterization of Bursicon Gene in Helicoverpa armigera (Lepidoptera: Noctuidae)
Xinjiang Agricultural Sciences,2020,Vol 57,No. 04
[Objective] Bursicon, a neuropeptitde hormone, consists of heterogenous proteins and plays an important role in the cuticle development and sclerotization of inscets. This study aims to obtain the cDNA sequences of two subunits (α subunit as burs and β subunit as pburs) of bursicon. We analyzed the molecular characteristics and expression pattern of bursicon in the cotton bollworm Helicoverpa armigera, in order to provide a foundation for further research on the functional mechanism. [Methods] The full-length cDNA sequence of bursicon gene was cloned from H. armigera by using bioinformatics and molecular cloning technique. The phylogenetic tree was constructed based on the amino acid sequences of burs and pburs of H. armigera and other known insects. Cluster analysis was operated with Jones Taylor Thornton (JTT) model of MEGA 7.0.14. The expression patterns of two genes at different developmental stages were respectively assayed with qRT-PCR. [Results] We cloned the full-length cDNA sequences of burs (GenBank accession number: AHM0247472.1) and pburs (GenBank accession number: AHM0247473.1) from H. armigera. The α subunit gene burs was 694 bp in length with a 480-bp open reading frame encoding 561 amino acid residues. Burs was highly expressed on day 3 of egg stage, day 1 of the 1st instar larval stage, day 1 of the 3rd–5th instar larval stage, 3rd–4th instar molting stages, and days 0, 3, and 7 of prepupal stage. The pburs is 779 bp in length with a 420-bp open reading frame encoding 139 amino acid residues. The β subunit (pburs) was highly expressed from egg to 3rd instar molting stage as well as on day 2 of the 3rd instar larval stage, day 1 of the 4th–5th instar larval stages, and day 1 of prepupal stage. In addition, burs and pburs had the highest expression levels in the thoracic ganalia, the second highest expression levels in the hypopharyngeal ganglia, and the lower expression levels in the brain and abdominal ganalia. [Conclusion] Bursicon is highly conserved in lepidopteran insects, and burs and pburs mainly act at the egg stage, early larva stage, pupa stage, and emerging stage. Bursicon is mainly synthesized by thoracic ganglia in the larva of H. armigera.
Study on Correlation Between the Plant Type Structure and Yield Components of Machine-picked Cotton Varieties in Southern Xinjiang
Xinjiang Agricultural Sciences,2020,Vol 57,No. 04
[Objective] To analyze the plant type structure and yield traits of different upland cotton varieties and to clarify the characteristics of plant type structure and yield components of high-yield and high-quality machine-picked cotton varieties. [Method] We determined the plant type traits and yield traits of different machine-picked cotton varieties. Based on significance analysis of difference and correlation analysis, we studied the characteristics of plant type, yield components, and plant type-yield correlation of several machine-picked cotton varieties. [Result] The seed cotton yield was in insignificantly positive correlation with the plant height, internode length of the main stem, number of fruit branches, and number of fruit nodes and in negative correlation with height of first node of fruit branch. The plant height was in significantly positive correlation with the height of first node of fruit branch and in extremely significant correlation with the internode length of the main stem. Variety 17N11 had pyramid structure, intact leaves at the base of the main stem, the highest yield, and the largest plant height. Variety 17N13 had the most leaves on the main stem and 17N5 had the smallest leaf abscission rate. For all the cotton varieties, the angle between the stem and fruit branches gradually increased from the top, while the variation showed the opposite trend (upper > middle > lower). The area of leaves on the main stem decreased from the top and the variation showed the opposite trend (upper > middle > lower). [Conclusion] High-yield varieties of machine-picked cotton should have the following traits: small angle between the stem and fruit branches (upper < 32.0°, middle < 50.0°), large total area of main stem leaves (> 320 cm 2), and small abscission rate of leaves at the middle and lower parts of the main stem (lower < 20%), which are the basis for selection of machine-picked cotton in plant type breeding.