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Xinjiang Agricultural Sciences aims to serve the agriculture and promote agricultural production in the arid areas, with highlights on the scientific and technological achievements and advanced technologies for agriculture in the arid areas.
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Editor-in-Chief: Cheng Zhili

Cloning and Molecular Characterization of Bursicon Gene in Helicoverpa armigera (Lepidoptera: Noctuidae)

MA Xingyu;JI Yujie;XUE Yuying;DU Mengfang;WEI Jizhen;YIN Xinming;LIU Xiaoguang;AN Shiheng

Xinjiang Agricultural Sciences,2020,Vol 57,No. 04

[Objective] Bursicon, a neuropeptitde hormone, consists of heterogenous proteins and plays an important role in the cuticle development and sclerotization of inscets. This study aims to obtain the cDNA sequences of two subunits (α subunit as burs and β subunit as pburs) of bursicon. We analyzed the molecular characteristics and expression pattern of bursicon in the cotton bollworm Helicoverpa armigera, in order to provide a foundation for further research on the functional mechanism. [Methods] The full-length cDNA sequence of bursicon gene was cloned from H. armigera by using bioinformatics and molecular cloning technique. The phylogenetic tree was constructed based on the amino acid sequences of burs and pburs of H. armigera and other known insects. Cluster analysis was operated with Jones Taylor Thornton (JTT) model of MEGA 7.0.14. The expression patterns of two genes at different developmental stages were respectively assayed with qRT-PCR. [Results] We cloned the full-length cDNA sequences of burs (GenBank accession number: AHM0247472.1) and pburs (GenBank accession number: AHM0247473.1) from H. armigera. The α subunit gene burs was 694 bp in length with a 480-bp open reading frame encoding 561 amino acid residues. Burs was highly expressed on day 3 of egg stage, day 1 of the 1st instar larval stage, day 1 of the 3rd–5th instar larval stage, 3rd–4th instar molting stages, and days 0, 3, and 7 of prepupal stage. The pburs is 779 bp in length with a 420-bp open reading frame encoding 139 amino acid residues. The β subunit (pburs) was highly expressed from egg to 3rd instar molting stage as well as on day 2 of the 3rd instar larval stage, day 1 of the 4th–5th instar larval stages, and day 1 of prepupal stage. In addition, burs and pburs had the highest expression levels in the thoracic ganalia, the second highest expression levels in the hypopharyngeal ganglia, and the lower expression levels in the brain and abdominal ganalia. [Conclusion] Bursicon is highly conserved in lepidopteran insects, and burs and pburs mainly act at the egg stage, early larva stage, pupa stage, and emerging stage. Bursicon is mainly synthesized by thoracic ganglia in the larva of H. armigera.

Study on Correlation Between the Plant Type Structure and Yield Components of Machine-picked Cotton Varieties in Southern Xinjiang

ZHENG Juyun;WANG Junduo;ZHANG Zekun;LIANG Yajun;GONG Zhaolong;AI Xiantao;GUO Jiangping;MO Ming;LI Xueyuan

Xinjiang Agricultural Sciences,2020,Vol 57,No. 04