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2020

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Brief

It aims to report the latest achievements in natural product research, develop a wide range of uses for natural product research, promote academic exchanges at home and abroad, strengthen the process of scientific research, development and production, and spread strategic, forward-looking and innovative scientific and technological achievements to promote the development of China's technology and economic.
Editorial Board

Editor-in-Chief: Li Bogang

Effect and mechanism of quercetin on blood pressure, intestinal flora, and ventricular remodeling in spontaneously hypertensive rats

ZHOU Xiu;LIU Jing;LI Jia-fu

Natural Product Research and Development,2020,Vol 32,No. 09

Screening of core genes and key microRNAs in the treatment of Helicobacter pylori-related peptic ulcer by Radix Scutellariae and Rhizoma Coptidis

CHEN Xin-yi;SONG Hou-pan;CHEN Xiao-juan;ZENG Mei-yan;YANG Tao;LIN Ye;LIU Heng-ming;FENG Yao

Natural Product Research and Development,2020,Vol 32,No. 09

The aim of this study was to explore the molecular network regulation mechanism of Rhizoma Coptidis and Radix Scutellariae (SRCR) on the treatment of Helicobacter pylori (Hp)-associated peptic ulcer (PU) on the basis of network pharmacology and bioinformatics methods, and to screen out the core genes and key microRNAs. Firstly, the main active ingredients and possible targets of SRCR were retrieved through the Chinese Medicine System Pharmacology Database and Analysis Platform (TCMSP). GSE70394 gene expression profile data were downloaded from the GEO database. The microarray data of differentially expressed genes in Hp-infected human gastric epithelial cells were homogenized by R software, and the volcano map and clustering heatmap of differentially expressed genes were plotted. Secondly, STRING database was used to construct the protein-protein interaction network between SRCR component target and Hp-infected human GES cells. The GO term enrichment and KEGG pathway enrichment analysis of the above-mentioned characteristic genes was performed with the DAVID database. Then, the core genes involved in the treatment of Hp-related PU by SRCR were screened out by Cytohubba plug-in unit. The key microRNAs regulating the core genes were predicted by the TargetScan and miRBase databases. Finally, 41 active components and 216 potential targets of SRCR were retrieved. The quality of GEO microarray data was high. With P < 0. 05 and |logFC| ≥ 2 as the screening conditions, 128 differentially expressed genes were obtained, including 77 up-regulated genes and 51 down-regulated genes. A total of 127 characteristic gene targets were further obtained for the treatment of Hp-related PU by SRCR. GO analysis of these targets showed that the biological processes involved 55 terms such as inflammatory response, and immune response. Cellular components included 11 terms such as extracellular space, exosomes, and nuclei. MFs included 15 terms such as growth factor activity and epidermal growth factor receptor binding. KEGG enrichment analysis results showed that 33 signaling pathways, including cytokine receptor interaction pathway, nod-like receptor signaling pathway, and NF- κB signaling pathway were closely related to the treatment of Hp-related PU by SRCR. Ten core genes regulated by SRCR, such as CXCL8, IL10, and IL1B were screened out by cytohubba. Ten of the most functional microRNAs, such as mir-93-5p, mir-374b-5p, and mir-3937 which may be regulated by SRCR, were further screened out. The above results indicate that the treatment of HpPU with SRCR has the characteristics of multi-component, multi-target, and multi-channel synergy. SRCR can exert its therapeutic effect on Hp-related PU by regulating the coding RNA (gene) and non-coding RNA (microRNA).