Sponsor(s): Chengdu Library and Information Center, Chinese Academy of Sciences
12 issues per year
Current Issue: Issue 04, 2020
It aims to report the latest achievements in natural product research, develop a wide range of uses for natural product research, promote academic exchanges at home and abroad, strengthen the process of scientific research, development and production, and spread strategic, forward-looking and innovative scientific and technological achievements to promote the development of China's technology and economic.
Editor-in-Chief: Li Bogang
Natural Product Research and Development,2020,Vol 32,No. 04
Human glioma U87 cells were treated with curcumin. The effect of curcumin on cell proliferation was assayed by CCK-8 method; the morphological changes of cells were observed; NADPH oxidase in cells was tested by ELISA. DCFH-DA fluorescence stain was used to detect the ROS level in cells. The oxidative stress indexes including total antioxidant capacity (T-AOC), malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione (GSH) were determined. Apoptosis was analyzed by AO/EB fluorescence staining and flow cytometry after double staining with annexin V/PI. Finally, the expression of proteins in apoptosis signaling pathway was detected by western blotting. The results showed that curcumin promoted ROS production by increasing cell NADPH oxidase activity, which decreased intracellular T-AOC, increased MDA content, decreased GSH content, and increased SOD activity. These results indicated curcumin induced oxidative stress in cells, possibly increased ROS to trigger the cell signaling pathway by down-regulating the expression of NF-κB/p65 protein, and finally promoted apoptosis through the apoptosis-executing molecule caspase-3.