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2020

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It aims to report the latest achievements in natural product research, develop a wide range of uses for natural product research, promote academic exchanges at home and abroad, strengthen the process of scientific research, development and production, and spread strategic, forward-looking and innovative scientific and technological achievements to promote the development of China's technology and economic.
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Editor-in-Chief: Li Bogang

Stasis-resolving collaterals-dredging herbs alleviate renal inflammation by inhibiting macrophage infiltration and activation via TAK1/JNK pathway in diabetic nephropathy rats

GUO Shuai;FANG Jing;LI Ya-chun;YANG Fan;CHEN Zhi-qiang

Natural Product Research and Development,2020,Vol 32,No. 12

To observe the effect of stasis-resolving collaterals-dredging herbs on renal inflammation in diabetic nephropathy (DN) rats and explore its mechanism. Ten SD rats were randomly selected as normal control group (C group), and the other 30 rats received one-time intraperitoneal injection of streptozotocin (STZ, 40 mg/kg) combined with high-glucose and high-fat diet to establish diabetes model. The successfully modeled rats were randomly classified into model group (M group) and stasis-resolving collaterals-dredging herb group (Z group). Z Group was treated with Chinese medicine. After 16 weeks, 24 h urine total protein (24 h UTP), monocyte chemotactic protein 1 (MCP-1), interleukin-1 β (IL-1 β), and tumor necrosis factor- α (TNF- α) were detected by ELISA. The expression of CD68 and inducible nitric oxide synthase (iNOS) was detected by immunofluorescence and the expression of TAK1/JNK pathway-related proteins transforming growth factor β-activated kinase 1 (TAK1), p-TAK1, c-Jun N-terminal kinase (JNK), and p-JNK was detected by Western blotting. The results are as follows. The levels of 24 h UTP, MCP-1, IL-1 β, and TNF- α were significantly increased ( P < 0.001), a large number of CD68 and iNOS protein deposits were seen in renal interstitium, and the expression of p-TAK1 and p-JNK protein was significantly increased in M group as compared with those in C group ( P < 0.001). The levels of 24 h UTP, MCP-1, IL-1 β, and TNF- α were significantly decreased ( P < 0.001, P < 0.001, P < 0.01, P < 0.01), the deposition of CD68 and iNOS protein was significantly decreased, and the expression of p-TAK1 and p-JNK protein was significantly decreased in Z group as compared with those in the M group ( P < 0.05). The results suggested that stasis-resolving collaterals-dredging herbs can reduce proteinuria and alleviate renal inflammation in DN rats, and its mechanism may be related to its reduction of macrophage infiltration and activation, and release of inflammatory factors in the renal tissue via inhibiting the TAK1/JNK pathway.

Study on the mechanism of emodin alleviating oxidative damage of mice diabetic nephropathy by regulating miR-21-mediated autophagy

QI Bao-ning;XIONG Yong-ai;PAN Yang-fang;XU Shou-zhu;JI Ming-rui;WANG Jia-xin;WANG Yi;ZOU Jia-ying

Natural Product Research and Development,2020,Vol 32,No. 12