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Issue 01
Brief

Founded in 1985,Chinese Journal of Biotechnology is the official journal of the Institute of Microbiology, the Chinese Academy of Sciences and the Chinese Society for Microbiology. It is an international,peer-reviewed journal that publishes original papers and reviews on all aspects of Biotechnology, such as genetic engineering,cell engineering, enzyme engineering, biochemical engineering, and so on.The journal is indexed/abstracted in various important citation resources such as Chemistry Abstracts,MEDLINE/PubMed,AJ OF vINITI, JST, Scopus, Center for Agriculture and Bioscience International, EMBASE, CNKI,CBM,Chinese Science and Technological Periodical(CD).
Editorial Board

Eidtor-in-chief
Shengli Yang

Associate Editors(alphabetically)
Guoqiang Chen, Huanchun Chen, Shouyi Chen, Zixin Deng, Rongxiang Fang

Board members(aiphabetically, *standing members)
Fengwu Bai, Yilin Cao, Hongzhang Chen, Jian Chen, Lin Chen, Sumin Chen

Comparison of immunogenicity between recombinant flagellins C and B of Salmonella abortus equi

WANG Hao;YI Yuanyang;DUISHANBAYI·Gulimire;FAN Qinggeng;SU Yan

Chinese Journal of Biotechnology,2020,Vol 36,No. 01

Establishment of RIG-I knockout 293T cell line and its effect on the replication of influenza B virus

TIAN Lu;JIAO Pengtao;HOU Lidan;LI Yun;SONG Zhengyu;LIU Wenjun;FAN Wenhui;SUN Lei

Chinese Journal of Biotechnology,2020,Vol 36,No. 01

The CRISPR/Cas9 gene editing technology directs Cas9 protein to recognize, bind and cleave the target site specifically by using artificial single-guide RNA(sgRNA), through non-homologous end joining or homologous end-recombinant repair mechanisms of cells, which can be engineered to knockout or knock-in of genomes. RIG-I is a pattern recognition receptor that recognizes the 5′-triphosphate-containing RNA in the cytoplasm and activates IRF3/7 and NF-κB by interacting with the downstream signaling molecule MAVS, thus initiating the expression of type I interferons and inflammatory factors. Previous studies found that influenza B virus (IBV) can up-regulate the expression of RIG-I. In the present study, to explore whether RIG-I is the major receptor for IBV to active the antiviral innate immune response and its effect on IBV replication, RIG-I gene in 293T cells was knocked out by CRISPR/Cas9 system, and a stable RIG-I knockout 293T ( RIG-I−/− 293T) cell line was screened by puromycin pressure. The results of Western blotting showed that RIG-I was not expressed in this cell line after IBV or Sendai virus(SeV)infection, indicating that the RIG-I−/− 293T cell line was successfully constructed. The transcription levels of interferons, inflammatory factors and interferon-stimulated genes in RIG-I−/− 293T cells which were infected by IBV decreased significantly compared with those in wild-type 293T cells. Moreover, the phosphorylation of p65 and IRF3 were not detected in IBV or SeV infected RIG-I−/− 293T cells. It is indicated that the expression of cytokines mainly depends on the RIG-I-mediated signaling pathway at the early stage of IBV infection. Furthermore, the multi-step growth curves of IBV in the wild-type and RIG-I−/− 293T cells showed that RIG-I inhibited the replication of IBV. Collectively, the RIG-I knockout 293T cell line was successfully constructed. We found that RIG-I is the main receptor for IBV to active the antiviral innate immune response and is critical for inhibiting IBV replication, which lays the foundation for further study of IBV infection mechanism.

Eukaryotic expression, protein purification and biological effects research of human CS1-Fc fusion protein

CHEN Ruzhang;WANG Xitong;LI Yanchen;GAO Jimin

Chinese Journal of Biotechnology,2020,Vol 36,No. 01