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Brief

Founded in 1985,Chinese Journal of Biotechnology is the official journal of the Institute of Microbiology, the Chinese Academy of Sciences and the Chinese Society for Microbiology. It is an international,peer-reviewed journal that publishes original papers and reviews on all aspects of Biotechnology, such as genetic engineering,cell engineering, enzyme engineering, biochemical engineering, and so on.The journal is indexed/abstracted in various important citation resources such as Chemistry Abstracts,MEDLINE/PubMed,AJ OF vINITI, JST, Scopus, Center for Agriculture and Bioscience International, EMBASE, CNKI,CBM,Chinese Science and Technological Periodical(CD).
Editorial Board

Eidtor-in-chief
Shengli Yang

Associate Editors(alphabetically)
Guoqiang Chen, Huanchun Chen, Shouyi Chen, Zixin Deng, Rongxiang Fang

Board members(aiphabetically, *standing members)
Fengwu Bai, Yilin Cao, Hongzhang Chen, Jian Chen, Lin Chen, Sumin Chen

Development, identification and application of 33 monoclonal antibodies against cardiac troponin T

Hu Yuehong ;Chen Zimin ;Chen Yuxiang ;Yang Yinghui ;Wei Shuying ;Liuwei Song;Zhou Guoliang ;Ge Shengxiang

Chinese Journal of Biotechnology,2016,Vol 32,No. 12

Construction of Spider Silk Functional Platform via Intein Trans-splicing

Senzhu Lin;Gefei Chen;Qing Meng

Chinese Journal of Biotechnology,2016,Vol 32,No. 12

Construction and Characterization of Type Ⅲ Secretion System of Attenuated Salmonella typhimurium

Chuan Yu;Chongkai Zhai;Chengshui Liao;Zuhua Yu;Lei He;Yanyan Jia;Jing Li;Chunjie Zhang;Xiangchao Cheng

Chinese Journal of Biotechnology,2016,Vol 32,No. 12

In order to develop a recombinant attenuated Salmonella typhimurium as oral live vaccine vector, we constructed recombinant plasmid pYA-sopENt100 by replacing the Ptrc promoter with the sopE promoter and secretion signal sequence sopENt100 of Salmonella typhimurium on the basis of plasmid pYA3493. Then, the complementary plasmid pYA-sopENt100 was transformed into ΔcrpΔasdSL1344 by electroporation to generate attenuated Salmonella typhimurium type Ⅲ secretion system ΔcrpΔasdSL1344 (pYA-sopENt100). We further characterized ΔcrpΔasd SL1344 (pYA-sopENt100). We also constructed a recombinant strain ΔcrpΔasdSL1344 (pYA-sopENt100-egfp) that harbored the reporter gene-enhanced green fluorescent protein (egfp) gene. Vero cells were infected with ΔcrpΔasdSL1344 (pYA-sopENt100-egfp) and the ability of delivery foreign antigens was tested via Western blotting analysis. The results of PCR, enzyme digestion and sequencing showed that the ΔcrpΔasdSL1344 (pYA-sopENt100) type Ⅲ secretion system was constructed successfully. The serotype of ΔcrpΔasdSL1344 (pYA-sopENt100) was identical to ΔcrpΔasdSL1344 and SL1344. Compared with wild strain SL1344, the biochemical characteristics of ΔcrpΔasdSL1344 (pYA-sopENt100) had obvious change, but it was basically the same with ΔcrpΔasdSL1344. The growth speed was much slower than that of the wild strain SL1344. The chicken virulence test (LD50) showed that the virulence of ΔcrpΔasd SL1344 (pYA-sopENt100) was 7 × 104 times lower than SL1344. In addition, we observed the 37 kDa sopENt100-egfp protein in the cultured supernatant of ΔcrpΔasdSL1344 (pYA-sopENt100-egfp) strain by Western blotting analysis. However, both the 37 kDa sopENt100-egfp protein and 27 kDa EGFP protein were detected in ΔcrpΔasdSL1344 (pYA-sopENt100-egfp)-infected Vero cells. These results demonstrated that the recombinant Salmonella typhimurium type Ⅲ secretion system ΔcrpΔasdSL1344 (pYA-sopENt100) was successfully constructed, and it should be used as a live vaccine vector for expressing foreign genes.

Establishment of L-periaxin Gene Knock-out RSC96 Cell Line

Min Liang;Tingting Peng;Yawei Shi

Chinese Journal of Biotechnology,2016,Vol 32,No. 12

Comparison of effects of staphylococcal nuclease A fused with different exogenous DNA fragments

Lixia Fu;Dejun Ji;Xubin Lu;Xian'gan Han;Wenzhi Wei

Chinese Journal of Biotechnology,2016,Vol 32,No. 12

Preparation and antioxidant activity detection of collagen peptide from Cirrhinus molitorella skin

Wu Cuiling ;Wu Ribang ;Liu Dan ;Yang Xinghao ;Zhang Jiang ;Huang Jiafeng;He Hailun

Chinese Journal of Biotechnology,2016,Vol 32,No. 12