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Brief

The aims of the Acta Entomologica Sinica are to report the most recent achievements and advances in entomological sciences, and to promote academic exchanges among entomological researchers in China and abroad. The journal publishes original research papers, review articles and short communications concerning various aspects of entomology and various groups of insects and mites, and accepts submissions in Chinese or English. Currently, original research papers are organized in four regular subsections, i.e., Physiology and Biochemistry, Toxicology and Resistance, Ecology and Pest Management, and Evolution and Systematics, and irregular subsections as necessary, such as Pathology and Medical Insects, for each issue.
Editorial Board

Editor-in-Chief：Sun Jianghua

Executive editor-in-chief: YUAN Decheng

Associate Editor
Gao Xiwu, Liu Shusheng, Wan Fanghao, Xia Qingyou, Xu Weihua, Zhao Xiaofan

Differential expression of multicopper oxidase 4 gene and biological effects after RNAi and feeding on different rice varieties in Sogatella furcifera (Hemiptera: Delphacidae)

JIA Hao-Kang;LIU Yu-Di;HOU Mao-Lin

Acta Entomologica Sinica,2019,Vol 62,No. 12

Screening of reference genes for quantitative real-time PCR in Lygus pratensis (Hemiptera: Miridae)

JIA Bing;MA Yi;PANG Bao-Ping;SHAN Yan-Min;BAO Qing-Long;HAN Hai-Bin;TAN Yao

Acta Entomologica Sinica,2019,Vol 62,No. 12

[Aim] To screen out the most stably expressed reference genes in Lygus pratensis under specific conditions. [Methods] qRT-PCR was adopted to determine expression levels of 10 candidate reference genes including alpha-tubulin, beta-tubulin, actin, elongation factor, succinate dehydrogenase, ubiquitin, transcription initiation factor TFIID subunit, glutathione S-transferase, ribosomal protein L32, and TATA-box binding protein genes in six categories of samples of L. pratensis including adults of different genders, different adult tissues, different developmental stages, adults with different lambda-cyhalothrin resistance, and adults respectively exposed to different temperatures and different insecticides. BestKeeper, geNorm, NormFinder, and RefFinder were adopted to analyze the mRNA expression stability of the candidate reference genes. [Results] According to the results of different analysis methods based on the effective qRT-PCR data, it was concluded that the most stably expressed reference gene was RPL32 for different adult tissues and adults of L. pratensis with different lambda-cyhalothrin resistance. The most stably expressed reference genes for the groups of adults exposed to different temperatures, adults of different genders, adults exposed to different insecticides and different developmental stages were UBQ, SDHA, β-tubulin and TAF, respectively. Based on the comprehensive analysis from geNorm and RefFinder calculation, the best combinations of stably expressed reference genes for the groups of different adult tissues, adults of different genders, different developmental stages, adults with different lambda-cyhalothrin resistance, and adults exposed to different temperatures and different insecticides were RPL32 + TAF, SDHA + GST, TAF + GST + UBQ, RPL32 + GST, UBQ + ACT + β-tubulin, and β-tubulin + TAF + RPL32, respectively. [Conclusions] All the most stably expressed reference genes and their best combinations obtained from this study could be used as reference genes for the expressiion analysis of genes in L. pratensis for future research.