Publisher(s): China Academic Journals (CD Edition) Electronic Publishing House Co., Ltd.
ISBN: ISBN 978-7-499-00978-3 pdf
First Published: 2020.11.23
Discipline(s): Medicine & Public Health
Price: $109 (for individuals) (中国大陆地区个人用户点此直接购买)
Institution users and community members, please click here to contact us.
Inflammation, as part of China’s Medicine Progress Series, has 76 excellent articles on inflammation research. These articles included were recommended by the editorial boards of the journals in which they were originally published, covering theoretical, drug, mechanism, clinical, and nursing research and fully demonstrating the cutting-edge advances in inflammation treatment in China, with methodological support and objective conclusions. The original articles are published in Chinese, and this book is a compilation of English versions of selected articles.
LIU Changxiao is the honorary president, tenured principal scientist and academic committee director of Tianjin Institute of Pharmaceutical Research, and the director of the State Key Laboratory of Drug Delivery Technology and Pharmacokinetics, the Center
1. Comparison of the effect between electroacupuncture and NSAIDs on pain memory based on cAMP/PKA/CREB pathway in anterior cingulate gyrus
Chinese Acupuncture & Moxibustion,Part 4: Clinical Study,Vol 40,No. 01
Objective To observe the direct intervention effects of electroacupuncture (EA) and non-steroid anti-inflammatory drugs (NSAIDs) on pain memory, and to explore their effects on cAMP/PKA/cAMP pathway in anterior cingulate gyrus (ACC). Methods Fifty clean healthy male SD rats were randomly divided into a control group, a model group, an indomethacin group, an EA group and a sham EA group, 10 rats in each group. Except the control group, the pain memory model was established in the remaining four groups by twice injection of carrageenan at foot; 0.1 mL of 2% λ-carrageenan was subcutaneously injected at the left foot of rats; 14 days later, when the pain threshold of rats of each group returned to the basic level, the second injection was performed with the same procedure. The rats in the EA group were treated with EA at bilateral Zusanli (ST36) for 30 min; the rats in the indomethacin group were treated with indomethacin intragastric administration with the dose of 3 mg/kg; the rats in the sham EA group were treated with EA without electricity at the point 0.3 mm forward Zusanli (ST36) with the depth of 2 mm for 30 min; the rats in the control group were not given any invention. All the above interventions were performed 5 h, 1 d, 2 d and 3 d after the second injection of 2% λ-carrageenan. The left paw withdrawal thresholds (PWT) were observed before the first injection, 4 h, 3 d, 5 d after the first injection, before the second injection and 4 h, 1 d, 2 d and 3 d after the second injection. Three days after the second injection, the number of positive cells of cAMP, p-PKA, p-CREB and the number of positive cells of protein co-expression in the right ACC brain area were detected by immunofluorescence, and the relative protein expression of p-PKA and p-CREB were detected by Western blot. Results Compared with the control group, the PWTs in the model group decreased significantly 4 h, 3 d and 5 d after the first injection and 1 d, 2 d and 3 d after the second injection ( P < 0.05); compared with the control group, the positive expression of cAMP, p-PKA and p-CREB in the right ACC brain area in the model group increased significantly ( P < 0.05), and the number of positive cells of the co-expression of cAMP/p-PKA and p-PKA/p-CREB also increased significantly ( P < 0.05). Compared with the model group, indomethacin group and sham EA group, the PWTs in the EA group were increased significantly 1 d, 2 d and 3 d after the second injection ( P < 0.05); compared with the model group, indomethacin group and sham EA group, the positive expression of p-PKA and p-CREB in the right ACC brain area in the EA group decreased significantly ( P < 0.05), and the number of positive cells of co-expression of cAMP/p-PKA and p-PKA/p-CREB was decreased significantly ( P < 0.05). Compared with the model group and sham EA group, the positive expression of cAMP in the right ACC brain area was decreased in the EA group ( P < 0.05). Conclusion EA have a direct intervention effect on pain memory, which has significant advantage over NSAIDs in the treatment of chronic pain. The advantage effect of EA on pain memory may be related to the inhibition of cAMP/PKA/CREB pathway in ACC area.
2. Effect of Chuanhuang No. 1 Recipe on Renal Function and Micro-inflammation in Phase 3 Chronic Kidney Disease Patients
Chinese Journal of Integrated Traditional and Western Medicine,Part 4: Clinical Study,Vol 35,No. 02
Objective To observe the effect of Chuanhuang No. 1 Recipe (CHR) on renal function and micro-inflammation in phase 3 chronic kidney disease (CKD) patients. Methods Totally 60 phase 3 CKD patients were randomly assigned to the treatment group (treated by CHR) and the control group (treated by Losartan Potassium), 30 in each group. All patients received basic treatment. Patients in the treatment group took CHR decoction, 400 m L each time, one dose per day, while those in the control group took Losartan Potassium, 50- 100 mg per day. All medication lasted for 24 weeks. Changes of serum creatinine (SCr), blood urea nitrogen (BUN), estimated glomerular filtration rate (e GFR), serum uric acid (UA), 24 h urinary protein excretion (24 h U-pro), urinary microalbumin (U-Alb), high-sensitivity C-reactive protein (hs-CRP), serum tumor necrosis factor (TNF)-α, and serum IL-6 were detected and compared before and after treatment. Efficacy was also compared. Results Compared with before treatment, SCr and BUN significantly decreased in the treatment group ( P < 0.05, P < 0.01); e GFR increased ( P < 0.05). Only UA obviously decreased in the control group ( P < 0.05), but with no obvious change in SCr, BUN, or e GFR. Compared with before treatment, 24 h U-pro decreased after treatment in the treatment group ( P < 0.05), but with less decreased level when compared with the control group. U-Alb was also significantly decreased in the control group ( P < 0.01). There was statistical difference in 24 h U-pro and U-Alb between the two groups after treatment ( P < 0.05). Compared with before treatment, hs-CRP obviously decreased after treatment in the two groups, but serum levels of TNF-α and IL-6 obviously decreased only in the treatment group ( P < 0.05). The total effective rate was obviously higher in the treatment group than in the control group (70.00% vs 43.33%, P < 0.01). Conclusion CHR could efficiently improve the renal function of phase 3 CKD patients and alleviate the micro-inflammation.
3. Efficacy of Chinese Herbs in Multiple Paths for Tubal Factor Infertility Patients and Their Effect on Serum Inflammatory Factors
Chinese Journal of Integrated Traditional and Western Medicine,Part 4: Clinical Study,Vol 36,No. 03
Objective To evaluate the clinical efficacy of Chinese herbs in multiple paths for tubal factor infertility (TFI) patients, and to observe their effect on serum inflammatory factors. Methods Totally 100 TFI patients were assigned to the observation group and the control group according to admission sequence, 50 in each group. All patients received laparoscopy. Patients in the observation group were additionally combined with traditional Chinese herbal treatment program in multiple paths (oral administration of Chinese herbs + retention enema of Chinese herbs + iontophoresis). All treatment lasted for three successive months. Scores of Chinese medicine (CM) symptoms, clinical efficacy, pregnancy rate, and levels of interleukin-6 (IL-6) and tum or necrosis factor-α (TNF-α) were compared between the two groups after six months of treatment. Results Scores of CM symptoms were significantly lower in the two groups after treatment ( P < 0.05). They were lower in the observation group than in the control group ( P < 0.05). Serum levels of TNF-α and IL-6 were significantly lower in the observation group than in the control group, with statistical difference ( P < 0.05). The effective rate was 96.0% (48/50) in the observation group and 82.0% (41/50) in the control group, with statistical difference ( χ 2 = 5.005, P < 0.05). After one year of postoperative follow-up, the intrauterine pregnancy rate was 58.0% (29/50) in the observation group and 34.0% (17/50) in the control group, with statistical difference ( χ 2 = 5.797, P < 0.05). The ectopic gestation occurred to one patient of the control group, and none of the observation group, with no statistical difference in the ectopic gestation rate between the two groups ( χ 2 = 1.010, P > 0.05). Conclusion Chinese herbs in multiple paths for treating TFI could significantly improve clinical symptoms, reduce expression of serum inflammatory factors, and elevate efficacy and pregnancy rate, which showed superiority when compared with laparoscopy alone.
4. Intervention of Qingshen Granule for Immune Inflammation of Chronic Renal Failure Patients with Damp-heat Syndrome
Chinese Journal of Integrated Traditional and Western Medicine,Part 4: Clinical Study,Vol 36,No. 04
Objective To observe the effect of Qingshen Granule (QG) on the immune function of chronic renal failure (CRF) patients with damp-heat syndrome (DHS).Methods A total of 162 CRF patients were assigned to the treated group and the control group by random digit table,81 in each group.All patients were treated with conventional Western medical therapy.Those in the treated group additionally took QG,one package each time (10 g),thrice per day.The therapeutic course for all was 12 weeks.The clinical efficacy was observed in the two groups.The contents of peripheral blood CD4 +T cells,CD8 +T cells,Th17 cells,and nuclear factor-κB p65 (NF-κB p65) activity were detected using flow cytometry.Expressions of serum IL-17,tumor necrosis factor receptor-associated factor 6 (TRAF6),matrix metalloproteinase-9 (MMP-9),matrix metalloproteinase inhibitor-1 (TIMP-1),collagen Ⅳ (Col-Ⅳ)were detected using ELISA.Results Finally 156 patients completed the trial (77cases in the treatment group and 79 cases in the control group).The total clinical curative effective rate was significantly higher in the treated group (80.52%,62/77) than in the control group (68.35%,54/79) with statistical differences between the two groups ( χ 2=54.849, P<0.05).Compared with before treatment in the same group,the levels of peripheral blood CD4 +/CD8 +,Th17 cell content, NF-κB p65 activity,serum levels of IL-17,TRAF6, and TIMP-1, TIMP-1/MMP-9 ratio, Col-Ⅳ level all decreased in the treated group after treatment ( P<0.05);serum MMP-9level decreased ( P<0.05) and TIMP-1/MMP-9 ratio increased ( P<0.05) in the control group.Compared with the control group,CD4 +/CD8 +T cell ratio,Th17 cell content,NF-κB p65 activity decreased more obviously in the treated group after treatment ( P<0.05).Serum levels of IL-17,TRAF6,TIMP-1,TIMP-1/MMP-9 ratio,and Col-Ⅳ all decreased ( P<0.05) and MMP-9 level increased ( P<0.05) in the treated group ( P<0.05).Conclusion QG could adjust immune dysfunction and disarranged immunity mediated inflammatory response,and attenuate renal fibrosis in CKD patients with DHS.
5. Real world feature analysis of Qingkailing Injection in combined medication in treating abnormal inflammatory factors
China Journal of Chinese Materia Medica,Part 4: Clinical Study,Vol 42,No. 05
Real world feature analysis was performed to investigate the combined medication with Qingkailing Injection in treating abnormal inflammatory factors such as elevated white blood cells (WBC) and C reactive protein (CRP). The patients of abnormal C reactive proteins and abnormal white blood cells treated with Qingkailing Injection were extracted from hospital information system ( HIS) of 16 Class 3A hospitals established by Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences. Then the basic information, traditional Chinese medicine and Western medicine diagnosis, prescription, and laboratory tests were analyzed. Apriori algorithm was used to construct the models and Clementine 12.0 for correlation analysis to analyze the clinical medication patterns and features of combined medication in patients with elevated WBC and CRP. The results showed that, when Qingkailing Injection was combined with one kind of western medicine or traditional Chinese medicine (TCM) in treating patients with abnormal CRP, vitamin C (159 cases, 74.30%) and Tanreqing Injection (71 cases, 33. 18%) were the most frequently used. When combined with two kinds of TCM, Xueshuantong Injection plus Tanreqing Injection (support degree: 10.75%) were most frequently used. When Qingkailing Injection was combined with one kind of western medicine or TCM in treating patients with abnormal WBCs, vitamin C (596 cases, 56.02%) and Ganmao Qingre granules (247 cases, 23.21%) were the most frequently used. When combined with two kinds of TCM, Shuanghuanglian + Ganmao Qingre granules (support degree 5.26%) were the most frequently used. In the patients with abnormal CRP and WBCs, its combinations with antibiotics and nutritional support agents were most common from the pharmacological perspective, indicating that in treating abnormal CRP, WBCs and other increased inflammatory indicators, Qingkailing Injection was most frequently combined with antibiotic drugs to achieve synergistic effect.
6. Effect of Internal and External Treatment of Osteoarthritis with Chinese Medicinal on Immune Inflammation Index Using Association Rules
Chinese Archives of Traditional Chinese Medicine,Part 4: Clinical Study,Vol 35,No. 06
Objective: To analyze the effect of Chinese medicinal and external drugs by Professor LIU Jian on immune inflammation index in osteoarthritis by using association rules and statistical methods, thereby providing the evidence for optimizing prescriptions. Methods: The data of hospitalized osteoarthritis patients in the Department of Rheumatology, The First Affiliated Hospital of Anhui University of Chinese Medicine from June, 2012 to October, 2015 were analyzed. We divided them into internal treatment group and combined internal and external treatment group according to whether the external treatment was used. SPSS 22.0 statistical analysis was carried out on the related indexes of the two groups. The relationship of Chinese medicinal and external drugs with immune inflammation index was analyzed by the Aprior module in SPSS Clementine 12.0. Results: There were 1 414 cases with osteoarthritis in this study, with 670 (47.3%) in the internal treatment group and 744 (52.6%) in the combined internal and external treatment group. Chinese medicinal was mainly classified into the four groups with the effect of clearing heat and removing toxicity, invigorating spleen for eliminating dampness, dispelling pathogenic wind and eliminating dampness, and promoting blood circulation for removing obstruction in collaterals, respectively. External therapy was mainly the topical application of Chinese medicinal, such as Hibiscus Paste and Stasis-Alleviating and Bone-Reuniting Powder. Compared with before treatment, internal treatment and combined internal and external treatment both could decrease the levels of complement C4, IgA, erythrocyte sedimentation rate (ESR), high-sensitivity C-reactive protein (hs-CRP) ( P < 0.05 or P < 0.01). Besides, the combined internal and external treatment can reduce the level of IgG and increase the level of superoxide dismutase (SOD). Compared with the internal treatment, the effect of combined internal and external treatment on reducing the levels of IgA, ESR, hs-CRP and increasing the level of SOD was better ( P < 0.05 or P < 0.01). With the confidence of 75% and the support of 20% under association rules, the Chinese medicinal Semen Coicis, Poria, aboveground part of S. orientalis, Caulis Spatholobi, Herba Taraxaci and Radix Cyathulae and Radix Angelicae Pubescentis had high association with the indexes. The external drugs Hibiscus Paste and Stasis-Alleviating and Bone-Reuniting Powder were highly associated with the related indexes. Conclusion: The internal Chinese medicinal for the treatment of osteoarthritis shall mainly have the effect of clearing heat and removing toxicity, invigorating spleen for eliminating dampness, dispelling pathogenic wind and eliminating dampness, and promoting blood circulation for removing obstruction in collaterals. Hibiscus Paste and Stasis-Alleviating and Bone-Reuniting Powder should be mainly used for the external treatment of osteoarthritis. The combined internal and external treatment was significantly more effective in improving the in vivo immuno-inflammatory reactions and upregulating the free radical scavenger SOD. Its action mechanism needs to be further studied.