Publisher(s):China Academic Journals (CD Edition) Electronic Publishing House Co., Ltd.
ISBN:ISBN 978-7-499-00963-9 pdf
First Published: 2020.11.23
Discipline(s): Medicine & Public Health
Price: $49 (for individuals) (中国大陆地区个人用户点此直接购买)
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Heart Disease (TCM), as part of China’s Medicine Progress Series, has 26 excellent articles on heart disease research with TCM. These articles fully demonstrate the unique therapies and good efficacy of TCM in treating heart diseases, with scientific methodological support and objective conclusions. The original articles were published in Chinese, and this book is a compilation of English versions of the selected articles.
Doctor LIU Jianxun, doctoral supervisor, Qihuang Scholar, principal investigator of China Academy of Chinese Medical Science, and principal scientist of the National Key Basic Research Program (973 Program), has presided over 20 national projects includin
1. Establishment of coronary heart disease models of Qi-deficiency and blood-stasis syndrome in rats
China Journal of Chinese Materia Medica,Part 2: Mechanism Study,Vol 41,No. 01
To establish Qi-deficiency and blood-stasis syndrome type coronary heart disease models by fatigue running exercise and high ligation of left anterior descending coronary artery (LAD), male Wistar rats were selected and randomly divided into sham operation group (JSS), coronary ligation group (DZ), fatigue running exercise + coronary ligation group (PZ). Coronary ligation alone was done in DZ group; while the rats in PZ group had running exercise in on the animal treadmill system for 2 weeks to establish fatigue models, and then coronary ligation was done based on the models. The exhausted running was maintained for 28 days at the frequency of 1 time/2 days after operation. Twenty-eight to thirty-one days after the operation, all the rats were observed for macroscopic physical signs, and the ultrasonic echocardiography indexes and breathing extent of the rats were collected to evaluate the main symptoms of rats with Qi-deficiency and blood-stasis syndrome type coronary heart disease; the related indexes of open field test, exhaustive running time, and colorimetric analysis data on images of plantar were collected to evaluate the accompanied symptoms; the colorimetric analysis data on lingual surface were collected to evaluate the tongue characteristics; the pulse distension data were collected to evaluate the pulse condition, and meanwhile, the blood rheology and coagulation function were also detected. From the 28 th day postoperatively, the main symptoms, accompanied symptoms, tongue characteristics and pulse conditions of rats in PZ group conformed to the symptoms of coronary heart disease and Qi-deficiency and blood-stasis syndrome. Combined with related pathological results, the study revealed that the Qi-deficiency and blood-stasis syndrome type coronary heart disease models could be successfully established by fatigue running exercise and high ligation of left anterior descending coronary artery for the rats.
2. Comparison of 4 types of coronary heart disease model of qi-deficiency and blood-stasis syndrome in rats
China Journal of Chinese Materia Medica,Part 2: Mechanism Study,Vol 41,No. 02
This paper was aimed to establish a method for coronary heart disease (CHD) in rats with Qi-deficiency and blood stasis by comparing different model establishment methods (L group: ligation of coronary artery, EL group: combination of exercise fatigue and ligation of coronary artery, DL group: combination of diet and ligation of coronary artery, DEL group: combination of diet, exercise fatigue and ligation of coronary artery). After 6 weeks post-operation, the method of L (ligation of coronary artery) and DL, EL, DEL (multi-originated information complex) were used to successfully establish CHD model of qi-deficiency and blood-stasis syndrome type in rats. Model set up by using the compound factors (DL, EL, DEL) and that through simple ligation of coronary artery (L) were used to compare the clinical etiology and the link. DL, EL and DEL were more closely with relevant theoretical system of traditional Chinese medicine (TCM) and have a certain advantage in completely reflect the characteristics of TCM syndrome. Among three kinds of compound factors models, EL model was more consistent with clinical practical reasons and characteristics of the disease than DL and DEL models. Through EL the CHD deficiency of blood stasis rat model of combined disease could be controlled and good repeated.
3. Effect of Yixintai Granule on mRNA and Protein Expression Levels of AQP 2 in Renal Medulla of Chronic Heart Failure Rabbits
Chinese Journal of Integrated Traditional and Western Medicine,Part 2: Mechanism Study,Vol 35,No. 03
Objective To explore the effect of Yixintai Granule (YG) on mRNA and protein expression levels of AQP 2 in renal medulla of chronic heart failure (CHF) rabbits. Methods CHF rat model was established by ear marginal vein injection of Adriamycin. Successfully modeled rabbits were divided into the model group, the high (8.4 g/kg), middle (4.2 g/kg), and low dose (2.1 g/kg) YG group, and the Furosemide group (2 mg /kg). Besides, a normal control group was set up. Equal volume of physiological saline was administered to rabbits of the model group and the normal control group by gastrogavage. YG at different doses was administered to rabbits of the 3 YG groups by gastrogavage. The intervention lasted for 4 weeks, once per day. After treatment the urine volume and pathomorphological changes of renal medulla tissue were observed. mRNA and protein expression levels of AQP 2 were detected. Results Compared with the normal control group, the urine volume decreased significantly, and mRNA and protein expression levels of renal medulla AQP 2 increased significantly in the model group (all P < 0.01). Compared with the model group, the urine volume increased significantly, and mRNA and protein expression levels of renal medulla AQP 2 decreased significantly in all medicated groups (all P < 0.01). Compared with the low dose YG group, the urine volume significantly increased and the mRNA expression level of renal medulla AQP2 significantly decreased in the middle and high dose YG groups (all P < 0.01). The expression level of AQP 2 protein significantly decreased in the high dose YG group (P < 0.01). Pathological changes of the renal medulla were the most obviously seen in the model group. But they were alleviated to various degrees in all medicated groups. They were more obviously attenuated in the middle and high dose YG groups. Conclusion YG could improve CHF possibly through down-regulating mRNA and protein expression levels of AQP2 in renal medulla, and elevating the urine volume.
4. Effect of Zishen Huoxue Decoction on Myocardial Pathomorphology of Coronary Heart Disease Rats with Syndrome of Kidney Deficiency and Blood Stasis
Chinese Journal of Integrated Traditional and Western Medicine,Part 2: Mechanism Study,Vol 36,No. 04
Objective To observe the effect of Zishen Huoxue Recipe (ZHR) on pathomorphology in coronary heart disease (CHD) rats with syndrome of kidney deficiency and blood stasis (SDBSS). Methods Total 60 healthy Wistar rats were divided into the blank group, the model group, high-, middle- and low-dose ZHR groups according to random digit table, with 12 in every group. Myocardial ischemia SDBSS rat model was prepared by ligating the left anterior descending coronary artery and injecting hydrocortisone. ZHR physic liquor was administered to rats in high-, middle- and low-dose ZHR groups at the daily dose of 21.6, 10.8, 5.4 g/kg by gastrogavage for 7 successive days, while equal volume of pure water was administered to rats in the blank group and the model group by gastrogavage for 7 successive days. Heart of rats was collected for pathomorphological observation under light microscope. Results In the model group the myocardia fiber was swollen and deformed with widened space, loose and dropsy tissues. Blood vessels in myocardial mesenchymal were dilated, infiltrated with more inflammatory cells. Myocardial cells were markedly swollen, degenerated, or necrotic, with caryolysis or disappearance of partial nuclear. A large amount of collagen fibrous tissue became hyperplasia. Endocardial blood vessels were swollen and degenerated with infiltration of few inflammatory cells. Epicardium tissue and structure were destroyed and got hyperplasia. Swollen, degenerated, or necrotic vessels could be seen, with infiltration of more inflammatory cells and collagen deposition. Pathomorphological injuries were alleviated in each ZHR group. The higher ZHR concentration, the milder the injury degree of myocardial tissue, the more limited range of damage. Conclusion ZHR could attenuate pathomorphological injuries of myocardial ischemia rats with SDBSS and regulate myocardial function, thus improving myocardial ischemia in CHD rats with SDBSS.
5. Effect of ginseng total saponins on hemodynamics and caspase-3 expression of myocardial cells in rats with chronic heart failure
The Chinese Journal of Clinical Pharmacology,Part 2: Mechanism Study,Vol 32,No. 05
Objective: To study the effect of Ginseng total saponins (GS) on hemodynamics and caspase-3 expression of myocardial cells in rats with chronic heart failure. Methods: Fifty male Wistar rats were randomly divided into control group, model group, GS low group (GS-L), GS moderate group (GS-M) and GS high group (GS-H) with each group 10 rats. Rats in control group and model group were given distilled water 40 mL once daily. Rats in the GS groups were given GS 5, 20 and 80 mg·kg ?1 gavage qd for 4 weeks. The changes of blood flow and the concentrations of brain natriuretic peptide (BNP) and Ca 2+ in myocardium in rats were measured. Results: Compared with the control group, the model group and GS group were significantly lower in aspects of left ventricular systolic pressure (LVSP) and the maximum change rate of left ventricular pressure (?dp/dt max) ( P < 0.05). But GS group was significantly higher than the model group ( P < 0.05). Compared with the control group, the model group and GS group were significantly higher in aspects of left ventricular end-diastolic pressure (LVEDP) and + dp/dtmax ( P < 0.05). The GS group was significantly lower than the model group ( P < 0.05).Compared with control group, brain natriuretic peptide (BNP) and Ca 2+ in model group were significantly elevated ( P < 0.05); GS group was significantly lower than that of model group ( P < 0.05). The expression of Caspase-3 in the control and GS groups was significantly lower than that in the model group ( P < 0.05), but the GS group was significantly higher than that in the control group ( P < 0.05). Conclusion:GS can improve the hemodynamic parameters of heart failure rats and decrease the concentration of calcium ion in cardiac muscle cells. The mechanism may be related to the inhibition of Caspase-3 expression in cardiomyocytes.