Phosphorus distribution inside Chinese fir seedlings under different P supplies based on 32P tracer
(2.Chinese Fir Engineering Research Center, National Forestry and Grassland Administration, Fuzhou, China 350002)
【Abstract】 Aim The objective of this study was to determine the amount and distribution of exogenous phosphorus (P) in different organs, as well as their changes in Chinese fir (Cunninghamia lanceolata) under different P supply levels. The results could be used as scientific basis for selecting P-efficient genotypes. Methods The seedlings of two Chinese fir genotypes (M1 and M4), both with high P use efficiency, were treated with different P supply levels and quantified by using 32P isotope tracer for P distribution in different organs. The seedlings used in this study were selected by our team through previous research as the experimental materials. Important findings We found that the distribution of exogenous P in M1 and M4 was the highest in the roots and the lowest in the stems, and at an intermediate level in the needles. The 32P concentration of each organ under the same treatment was in the order: root > stem > needle on the horizontal projection plane. The exogenous P concentrations in the roots, stems, and needles of M1 and M4 under low-P treatment appeared lower than those under the high-P treatment. The blackening degrees of low-P image of roots, stems, and needles under the same treatment were also lower than those under high-P treatment. The concentrations of exogenous P in these organs under the low-P treatment were increased slowly, indicating that the low-P stress significantly affected the absorption and accumulation of P in the seedlings. The P distribution rates in the roots of M1 and M4 showed an initial decreasing and increasing later under low-P stress, while under the high-P treatment, the root P level was increased first and stabilized later. These findings indicated that M1 and M4 could adapt to external low-P stress through the redistribution of P within the plants by transferring P from roots to above-ground parts at the early stage under low-P stress. With the extension of stressing time, the P from above-ground parts was shifted to roots. However, the distribution of exogenous P in M1 and M4 was significantly different under the low P treatment. The distribution of exogenous P from the beginning to the end of M1 was greater in the roots than that in above-ground parts, while M4 showed a similar pattern at the early stage but a higher rate toward the above-ground parts later. This indicated that M1 had a higher degree of strengthening P circulation in vivo than M4 with low-P stress, i.e., the tendency of P transfer from above-ground parts to roots was stronger in M1 than in M4.
【Keywords】 Cunninghamia lanceolata; phosphorus distribution; 32P isotope; low-phosphorus stress; phosphorus use efficiency; autoradiography;
Abel S, Ticeoni CA, Delatorre CA (2002). Phosphate sensing in higher plants. Physiologia Plantnrum, 115, 1–8.
Bennetzen JL, Hake SC (2009). Handbook of Maize: It’s Biology. Springer, New York. 381–404.
Cao LW, Guo XS, Long ZP, Ma CM (2012). Changes of phosphorus nutrition on P accumulation, yield and quality of soybean. Soybean Science, 34, 458–462 (in Chinese).
Chao MN, Zhang ZY, Zhang JB, Song HN, Bu JJ, Niu FQ, Wang QL (2017). Preliminary study on adaptability of cotton varieties to phosphorus deficiency stress in hydroponic culture. Journal of Yangzhou University (Agricultural and Life Science Edition), 38, 99–104 (in Chinese).
Chen SS (2002). The water holding capacity and soil fertility in the mixed forest of Cunninghamia lanceolata and Altingia gracilides. Acta Ecologica Sinica, 22, 957–961 (in Chinese).
Chen YL, Li XL, Zhou XY (2006). Effect of phosphorus deficiency stress on growth of larch seedlings and activities of acid phosphatase in roots. Journal of Beijing Forestry University, 28 (6), 46–50 (in Chinese).
Chen ZY, Wu PF, Zou XH, Wang P, Ma J, Ma XQ (2016). Relationship between growth and endogenous hormones of Chinese fir seedlings under low phosphorus stress. Scientia Silvae Sinicae, 52 (2), 57–66 (in Chinese).
Doerner P (2008). Phosphate starvation signaling: A threesome controls systemic Pi homeostasis. Current Opinion in Plant Biology, 11, 536–540.
Guo ZH, He LY, Xu CG (2005). Uptake and use of sparingly soluble phosphorus by rice genotypes with different P-efficiency. Acta Agronomica Sinica, 31, 1322–1327 (in Chinese).
Hu XQ, Yang WP, Huang L, Mei PP, Meng L (2018). Absorption and distribution of nitrogen, phosphorus and potassium in Carthamus tinctorius L. Journal of Northwest AandF University (Natural Science Edition), 46 (7), 1–7 (in Chinese).
Jeschke WD, Kirekby EA, Peuke AD, Pate JS, Hartung W (1997). Effect of P deficiency on assimilation and transport of nitrate and phosphate in intact plants of castor bean (Ricinus communis L.). Journal of Experimental Botany, 48, 75–91.
Leng HN, Chen YT, Duan HP, Rao LB, Wang YJ, Hu YX (2009). Effect of phosphorus stress on the growth and nitrogen and phosphorus absorption and utilization efficiency of Liquidambar formosana from different provenances. Chinese Journal of Applied Ecology, 20, 754–756 (in Chinese).
Li H, Shen J, Zhang F, Clairotte M, Drevon JJ, Le cadre E, Hinsinger P (2008). Dynamics of phosphorus fractions in the rhizosphere of common bean (Phaseolus vulgaris L.) and durum wheat (Triticumtur gidumdurum L.) grown in monocropping and intercropping systems. Plant and Soil, 312, 139–150.
Liang CY, Liao H (2015). Molecular mechanisms underlying the responses of plant roots to low-P stress. Chinese Bulletin of Life Sciences, 27, 289–397 (in Chinese).
Liang X, Liu AQ, Ma XQ, Feng LZ, Huang YJ (2006). Comparison of the phosphorus characteristics of different Chinese fir clones. Journal of Plant Ecology (Chinese Version), 30, 1005–1011 (in Chinese).
Lin KM, Yu XT (2001). Land resilience and sustainable management of Cunninghamia lanceolata plantation. Chinese Journal of Eco-Agriculture, 9 (4), 39–42 (in Chinese).
Liu J, Lü JY, Li SR (1996). Studies of absorption and accumulation of 32P in Larix gmelinii seedling. Acta Botanica Boreali-Occidentalia Sinica, 16, 136–139 (in Chinese).
Ma XQ, Fan SW, Chen SS, Lin SJ (2003). Study on biomass productivity of Chinese fir plantations after successive planting. Scientia Silvae Sinicae, 29 (2), 78–83 (in Chinese).
Mi GH, Xing JP, Chen FJ, Liu XS, Liu Y (2004). Maize root growth in relation to tolerance to low phosphorus. Plant Nutrition and Fertilizer Science, 10, 468–472 (in Chinese).
Postma JA, Lynch JP (2011). Theoretical evidence for the functional benefit of root cortical aerenchyma in soils with low phosphorus availability. Annals of Botany, 107, 829–841.
Qiang JY (2004). Study of phosphate absorption and distribution characteristics in potato and bean by using 32P. Guihaia, 24, 52–54 (in Chinese).
Qiang JY, Wang J, Chen GH, Guo M, Zhang X (1997). Study on phosphorus metabolism of maize seedlings using 32P tracer. Journal of Yunnan Agricultural University, 12 (3), 169–172 (in Chinese).
Raghothama KG (1999). Phosphate acquisition. Annual Review of Plant Physiology and Plant Molecular Biology, 50, 665–693.
Ruan JY, Zhang FS, Wong MH (2000). Effect of nitrogen form and phosphorus source on the growth, nutrient uptake and rhizosphere soil property of Camellia sinensis L. Plant and Soil, 223, 63–71.
Sheng WT, Fan SH, Ma XQ (2005). Study on long-term productivity maintenance mechanism of Cunninghamia lanceolata plantation. Science Press, Beijing (in Chinese).
Shenoy V, Kalagudi G (2005). Enhancing plant phosphorus use efficiency for sustainable cropping. Biotechnology Advances, 23, 501–513.
Su SS, Li M, Wu PF, Zhang Y, Ma XQ (2017). Cloning and expression analysis of phosphate transporter gene ClPht1;1 in Cunninghamia lanceolata. Scientia Silvae Sinicae, 53 (5), 33–42 (in Chinese).
Su SZ, Wu FK, Liu D, Wu L, Gao SB (2013). Cloning and functional analysis of a phosphor transporter gene from a Phtl family of maize. Journal of Nuclear Agricultural Sciences, 27, 885–894 (in Chinese).
Vance CP, Uhde-Stone C, Allan DL (2003). Phosphorus acquisition and use: Critical adaptations by plants for securing a nonrenewable resource. New Phytologist, 157, 423–447.
Veneklaas EJ, Shane MW, White PJ (2012). Opportunities for improving phosphorus-use efficiency in crop plants. New Phytologist, 195, 306–320.
Wu PF, Ma XQ, Tigabu M, Chen W, Liu AQ, Pre CO (2011). Root morphological plasticity and biomass production of two Chinese fir clones with high phosphorus efficiency under low phosphorus stress. Canadian Journal of Forest Research, 41, 228–234.
Wu PF, Wang GY, Taimoor HF, Li Q, Zou XH, Ma XQ (2017). Low phosphorus and competition affect Chinese fir cutting growth and root organic acid concentration: Does neighboring root activity aggravate P nutrient deficiency? Journal of Soils and Sediments, 17, 2775–2785.
Wu WH (2003). Plant Physiology. Science and Technology Press, Beijing. 91–92 (in Chinese).
Wu YQ, Lin Q, Yan MJ, Zhang H, Chen ZC, Li XF (2017). Effect of phosphorus on growth and nutrient uptake of tomato. Chinese Agricultural Science Bulletin, 33 (9), 74–77 (in Chinese).
Xie YR, Zhou ZC, Liao GH, Jin GQ, Chen Y (2005). Difference of induced acid phosphate activity under low phosphorus stress of Pinus massoniana provenances. Scientia Silvae Sinicae, 41 (3), 58–62 (in Chinese).
Yang Q, Zhang Y, Zhou ZC, Feng ZP (2012). Root architecture and phosphorus efficiency of different provenance Pinus massoniana under low phosphorous stress. Chinese Journal of Applied Ecology, 23, 2339–2345 (in Chinese).
Yu XT (1993). Research and views on sustainable utilization of Chinese fir woodland. World Forestry Research, (2), 80–87 (in Chinese).
Yu XT (1996). Fir Cultivation. Fujian Science and Technology Press, Fuzhou (in Chinese).
Zhang AQ, He LY, Men YY, Zhao HE, Yang JF, Li DH (2008). Effect of phosphorus levels on growth and nutrient absorption of low-P tolerant maize seedlings. Chinese Journal of Applied and Environmental Biology, 14, 347–350 (in Chinese).
Zhang B, Qin L (2010). Plants tolerance to low phosphorus and its molecular basis. Molecular Plant Breeding, 8, 776–783 (in Chinese).
Zhang LH, Zhang H, Huang YF, Ye YL, Zhang ZS, Zhan ZL (2013). Effect of phosphorus application on soil available phosphorus and maize phosphorus uptake and yield. Chinese Journal of Eco-Agriculture, 21, 801–809 (in Chinese).
Zhang LM, HE LY, Li JS, Xu SZ (2004). Investigation of maize inbred lines on tolerance to low-phosphorus stress at seedling stage. Scientia Agricultura Sinica, 37, 1955–1959 (in Chinese).
Zhang YL, Wang JY, Ma XZ, Chen LJ (2009). Research progress on activating technology for increasing phosphate efficiency. Chinese Journal of Soil Science, 40, 194–202 (in Chinese).
Zhou YR, Chen ML (1996). Study on absorption and distribution of 32P in Hemerocallis citrina. Journal of Southwest University (Natural Science Edition), 18, 416–420 (in Chinese).
Zou XH, Wei D, Wu PF, Zhang Y, Hu YN, Chen ST, Ma XQ (2018). Strategies of organic acid production and exudation in response to low-phosphorus stress in Chinese fir genotypes differing in phosphorus-use efficiencies. Trees, 32, 897–912.
Zou XH, Wu PF, Chen NL, Wang P, Ma XQ (2015). Chinese fir root response to spatial and temporal heterogeneity of phosphorus availability in the soil. Canadian Journal of Forest Research, 45, 402–410.