Phosphorus distribution inside Chinese fir seedlings under different P supplies based on 32P tracer

CHEN Si-Tong1,2 ZOU Xian-Hua1,2 CAI Yi-Bing1,2 WEI Dan1,2 LI Tao1,2 WU Peng-Fei1,2 MA Xiang-Qing1,2

(1.Forestry College of Fujian Agriculture and Forestry University, Fuzhou, China 350002)
(2.Chinese Fir Engineering Research Center, National Forestry and Grassland Administration, Fuzhou, China 350002)

【Abstract】 Aim The objective of this study was to determine the amount and distribution of exogenous phosphorus (P) in different organs, as well as their changes in Chinese fir (Cunninghamia lanceolata) under different P supply levels. The results could be used as scientific basis for selecting P-efficient genotypes. Methods The seedlings of two Chinese fir genotypes (M1 and M4), both with high P use efficiency, were treated with different P supply levels and quantified by using 32P isotope tracer for P distribution in different organs. The seedlings used in this study were selected by our team through previous research as the experimental materials. Important findings We found that the distribution of exogenous P in M1 and M4 was the highest in the roots and the lowest in the stems, and at an intermediate level in the needles. The 32P concentration of each organ under the same treatment was in the order: root > stem > needle on the horizontal projection plane. The exogenous P concentrations in the roots, stems, and needles of M1 and M4 under low-P treatment appeared lower than those under the high-P treatment. The blackening degrees of low-P image of roots, stems, and needles under the same treatment were also lower than those under high-P treatment. The concentrations of exogenous P in these organs under the low-P treatment were increased slowly, indicating that the low-P stress significantly affected the absorption and accumulation of P in the seedlings. The P distribution rates in the roots of M1 and M4 showed an initial decreasing and increasing later under low-P stress, while under the high-P treatment, the root P level was increased first and stabilized later. These findings indicated that M1 and M4 could adapt to external low-P stress through the redistribution of P within the plants by transferring P from roots to above-ground parts at the early stage under low-P stress. With the extension of stressing time, the P from above-ground parts was shifted to roots. However, the distribution of exogenous P in M1 and M4 was significantly different under the low P treatment. The distribution of exogenous P from the beginning to the end of M1 was greater in the roots than that in above-ground parts, while M4 showed a similar pattern at the early stage but a higher rate toward the above-ground parts later. This indicated that M1 had a higher degree of strengthening P circulation in vivo than M4 with low-P stress, i.e., the tendency of P transfer from above-ground parts to roots was stronger in M1 than in M4.

【Keywords】 Cunninghamia lanceolata; phosphorus distribution; 32P isotope; low-phosphorus stress; phosphorus use efficiency; autoradiography;


【Funds】 National Natural Science Foundation of China (U1405211) Science and Technology Major Project of the Fujian Province (2018NZ0001-1)

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This Article



Vol 42, No. 11, Pages 1103-1112

November 2018


Article Outline


  • 1 Materials and methods
  • 2 Results and analysis
  • 3 Discussion
  • 4 Conclusions
  • References