A new technology of modulated Chl a fluorescence image: In vivo measurement of the PSII maximum photochemical efficiency and its heterogeneity within leaves

FAN Da-Yong1 FU Zeng-Juan1 XIE Zong-Qiang1 LI Rong-Gui1 ZHANG Shu-Min1

(1.State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing, China 100093)

【Abstract】The spatial photosynthetic heterogeneity within leaves is an important prerequisite for the studies on the photosynthetic model, the mechanism (s) of photoinhibition, light protection, etc. However, currently the in vivo measurement of the spatial photosynthetic heterogeneity within leaves is difficult. The present study improved the device assembled by Vogelmann and Evans (2002), thereby acquired the photosystem II (PSII) maximum photochemical efficiency (Fv/Fm) images within leaves. Finally, these images were processed and data of Fv/Fm and its spatial variations could be obtained, with the aid of Matlab software. Based on the innovative technique, an investigation of the effects of strong light on the Fv/Fm and its spatial heterogeneity within leaves was carried out. It was found that Fv/Fm within leaves was not homogonous. Strong light led to a general decrease of Fv/Fm (PSII photoinhibition) across leaf section, and the palisade tissue close to the epidermis layer had high tolerance to photoinhibition. Compared with control, short-term photoinhibition caused a larger spatial variation of Fv/Fm within leaves, which may be related to the chloroplast-avoidance response induced by high-fluence. On the contrary, long-term light inhibition led to a smaller spatial variation of Fv/Fm within leaves, indicating such mechanism was no longer effective. Compared to other types of chlorophyll fluoremeter, the device in the present study can in vivo obtain the panoramic picture of Fv/Fm within leaves, providing a powerful tool for the studies on the mechanism (s) attributed to the spatial heterogeneity of photosynthetic capacity of leaf, which is critical for the understanding on several hot spots in the research field of photosynthesis.

【Keywords】 Spinacia oleracea; chlorophyll fluorescence image; photoinhibition;

【DOI】

【Funds】 National Natural Science Foundation of China (31370424) Knowledge Innovation Project of Chinese Academy of Sciences (KZCX2-XB3-09)

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(Translated by CHENG QZ)

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This Article

ISSN:1005-264X

CN:11-3397/Q

Vol 40, No. 09, Pages 942-951

September 2016

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Article Outline

Abstract

  • 1 Materials and Methods
  • 2 Results and analyses
  • 3 Discussion
  • References