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清肺排毒汤治疗新型冠状病毒肺炎机制的网络药理学探讨

赵静1 田赛赛2 杨健2 刘剑锋3 张卫东1,2

(1.上海中医药大学交叉科学研究院, 上海 201203)
(2.海军军医大学药学院, 上海 200433)
(3.中国中医科学院中国医史文献研究所, 北京 100700)

【摘要】目的 收集清肺排毒汤(QFPDT)的主要活性成分、靶标,探讨其靶标与2019年新型冠状病毒疾病(COVID-19)之间的联系以及多成分、多靶标的治疗机制。方法 从ETCM、TCMID、NPASS数据库搜索QFPDT每味中药的归经、成分和靶标,用Cytoscape软件构建和分析网络,用DAVID和STRING对靶标进行功能富集分析。结果 QFPDT里的中药大多数归肺经,其790个潜在靶标中的232个与新型冠状病毒(SARS-CoV-2)的受体血管紧张素转化酶2(ACE2)是共表达的;靶标包含7个两两相互作用的核糖体蛋白;重要靶标富集在病毒感染和肺部损伤2大类疾病通路上,且与HIV病毒的6个蛋白具有密切的相互作用;重要靶标调控内分泌系统、免疫系统、信号转导、翻译等生物学过程的一系列信号通路。结论 QFPDT通过多成分、多靶标对机体起到整体调控作用。其首要作用部位是肺、其次是脾;它通过调控若干与ACE2共表达的蛋白、以及与疾病发生发展密切相关的一系列信号通路,起到平衡免疫、消除炎症的作用;它靶向病毒复制必需的蛋白—核糖体蛋白而抑制病毒m RNA翻译、并抑制与病毒蛋白相互作用的蛋白而起到抗病毒作用。

【关键词】 新型冠状病毒;2019年新型冠状病毒疾病;清肺排毒汤;网络药理学;抗病毒;血管紧张素转化酶;

【DOI】

Investigating the mechanism of Qing-Fei-Pai-Du-Tang for the treatment of Novel Coronavirus Pneumonia by network pharmacology

ZHAO Jing1 TIAN Sai-sai2 YANG Jian2 LIU Jian-feng3 ZHANG Wei-dong1,2

(1.Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai, China 201203)
(2.School of Pharmacy, Second Military Medical University, Shanghai, China 200433)
(3.Institute for the History of Chinese Medicine and Medical Literature, China Academy of Chinese Medical Sciences, Beijing, China 100700)

【Abstract】 Objective To collect main ingredients and targets of Qing-Fei-Pai-Du-Tang (QFPDT), and to investigate the relationship between the targets and novel coronavirus pneumonia (COVID-19) and the therapeutic mechanism of its multiple components on COVID-19 via multiple targets. Methods The meridian tropisms, compounds and targets of each herb in QFPDT were collected from ETCM, TCMID and NPASS databases. Cytoscape software was used to construct and analysis networks. DAVID and STRING were applied for functional enrichment analysis of targets. Results The herbs in QFPDT mainly acted on the lung meridian. Among QFPDT’s 790 putative targets, 232 were co-expressed with ACE2, the receptor of novel coronavirus (SARS-CoV-2). The targets included seven densely interacting ribosomal proteins. Important targets were enriched on two classes of disease pathways, namely, virus infection and lung injury. In addition, many targets interacted with six proteins of HIV virus. Important targets regulated a series of pathways responsible for translation, endocrine system, immune system, nervous system and signal transduction. Conclusion QFPDT’s chief targeting organ is the lung and the second is the spleen. By regulating a series of proteins co-expressed with ACE2 and a series of signaling pathways closely related to the occurrence and development of diseases, it plays a role in balancing immunity and eliminating inflammation. It may act as an antiviral agent by targeting ribosomal proteins that are necessary for viral replication, so as to inhibit viral mRNA translation and a group of proteins that interact with viral proteins.

【Keywords】 SARS-CoV-2; COVID-19; Qing-Fei-Pai-Du-Tang; network pharmacology; anti-virus; angiotensin-converting enzyme 2;

【DOI】

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    References

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ISSN:0253-2670

CN: 12-1108/R

Vol , No. 02, Pages

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Abstract

  • 1 Methods
  • 2 Results
  • 3 Discussion
  • References