Investigating the mechanism of Qing-Fei-Pai-Du-Tang for the treatment of Novel Coronavirus Pneumonia by network pharmacology
(2.School of Pharmacy, Second Military Medical University, Shanghai, China 200433)
(3.Institute for the History of Chinese Medicine and Medical Literature, China Academy of Chinese Medical Sciences, Beijing, China 100700)
【Abstract】 Objective To collect main ingredients and targets of Qing-Fei-Pai-Du-Tang (QFPDT), and to investigate the relationship between the targets and novel coronavirus pneumonia (COVID-19) and the therapeutic mechanism of its multiple components on COVID-19 via multiple targets. Methods The meridian tropisms, compounds and targets of each herb in QFPDT were collected from ETCM, TCMID and NPASS databases. Cytoscape software was used to construct and analysis networks. DAVID and STRING were applied for functional enrichment analysis of targets. Results The herbs in QFPDT mainly acted on the lung meridian. Among QFPDT’s 790 putative targets, 232 were co-expressed with ACE2, the receptor of novel coronavirus (SARS-CoV-2). The targets included seven densely interacting ribosomal proteins. Important targets were enriched on two classes of disease pathways, namely, virus infection and lung injury. In addition, many targets interacted with six proteins of HIV virus. Important targets regulated a series of pathways responsible for translation, endocrine system, immune system, nervous system and signal transduction. Conclusion QFPDT’s chief targeting organ is the lung and the second is the spleen. By regulating a series of proteins co-expressed with ACE2 and a series of signaling pathways closely related to the occurrence and development of diseases, it plays a role in balancing immunity and eliminating inflammation. It may act as an antiviral agent by targeting ribosomal proteins that are necessary for viral replication, so as to inhibit viral mRNA translation and a group of proteins that interact with viral proteins.
【Keywords】 SARS-CoV-2; COVID-19; Qing-Fei-Pai-Du-Tang; network pharmacology; anti-virus; angiotensin-converting enzyme 2;
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