A new alkaloid isolated from leaves of Carthamus tinctorius

ZHANG Dong-bo1 SUN Chen1 LIU Hong-bo1 ZHANG Yan-lei1 LI Shi-ying1 WANG Ming-geng2 REN Zhen-li2 SONG Zhong-xing1 TANG Zhi-shu

(1.Shaanxi Collaborative Innovation Center of Chinese Medicinal Resource Industrialization, Shaanxi Key Laboratory of New Drugs and Chinese Medicine Foundation Research, Shaanxi Rheumatism and Tumor Center of TCM Engineering Technology Research, Shaanxi University of Chinese Medicine, Xianyang, China 712083)
(2.Shangdong Buchang Pharmaceuticals Co., Ltd., Heze, China 274000)

【Abstract】 Objective To study the alkaloids from leaves of Carthamus tinctorius. Methods The alkaloids were isolated and purified by silica gel, MCI, Sephadex LH-20 column chromatographies, and semi-preparative HPLC, and their structures were elucidated by physical and spectroscopic analysis. Results A new β-carboline alkaloid, 4,9-dimethoxy-1-ethyl-β-carboline (1) along with one known analogue 4-methoxy-1-ethyl-β-carboline (2), were isolated from the leaves of C. tinctorius. Compounds 1 and 2 showed the cytotoxicity against HepG2 cell lines with IC50 values of (15.20 ± 0.58) μmol/L and (17.40 ± 0.33) μmol/L, respectively. Conclusion Compounds 1 and 2 are firstly obtained from Carthamus genus, and compound 1 is a new compound, named carthine A. Both compounds 1 and 2 exhibited cytotoxicity against HepG2 cell lines.

【Keywords】 Carthamus tinctorius L.; carthine A; 4-methoxy-1-ethyl-β-carboline; β-carboline alkaloids; cytotoxicity;

【DOI】

【Funds】 Shaanxi Natural Science Basic Research Foundation (2017SF-365) Special Funds of the Natural Science of Shaanxi Provincial Department of Education (16JK1196)

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This Article

ISSN:0253-2670

CN:12-1108/R

Vol 50, No. 01, Pages 22-24

January 2019

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Article Outline

Abstract

  • 1 Materials and instruments
  • 2 Extraction and isolation
  • 3 Structural identification
  • 4 Cytotoxicity
  • References