Chemical constituents of Lonicera japonica roots and their anti-inflammatory effects

YU Jin-qian1 WANG Zhao-ping2 ZHU Heng2 LI Gang2 WANG Xiao1,2

(1.Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Jinan, China 250014)
(2.College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, China 250014)

【Abstract】To study the chemical composition and their anti-inflammatory activities of honeysuckle (Lonicera japonica Thunb.) roots, seventeen compounds were isolated from the roots of L. japonica Thunb. by various chromatography, including silica gel, Sephadex LH-20 and preparative HPLC. Their structures were identified by MS, IR, and nuclear magnetic resonance spectra, as 1-oxo-(1H)-cyclopenta [b] benzofuran-7-carbaldehyde (1), 4-hydroxycinnamic acid (2), chlorogenic acid (3), loganin aglycone (4), caffeic acid (5), secologanin dimethyl acetal (6), korolkoside (7), coniferin (8), sweroside (9), secoxyloganin (10), 5-O-caffeoylquinic acid (11), chlorogenic acid methyl ester (12), chlorogenic acid ethyl ester (13), 3,5-O-dicaffeoylquinic acid (14), 4,5-O-dicaffeoylquinic acid (15), grandifloroside (16), and 4,5-O-dicaffeoylquinic acid (17). Among those, compound 1 is a new compound, and compound 8 is found in L. japonica for the first time. Compounds 1, 3, 14–17 showed significant anti-inflammatory activities against macrophage in zebrafish.

【Keywords】 roots of Lonicera japonica; chemical constituent; identification; zebrafish; anti-inflammatory;


【Funds】 National Natural Science Foundation of China (81473298) Science and Technology Development Program of Shandong Province, China (2014GSF119031, 2014GZX219003).

Download this article


    [1]Zhang TT, Zhang YY, Chen HG, et al. Establishment of a novel preliminary screening model for bioactive parts of Lonicerae japonicae thumb. based on microcalorimetry[J]. Pharm J CPLA, 2011, 27: 205-207+211 (in Chinese).

    [2]Lei L, Li XP, Bai XL, et al. Study on anti-endotoxin, antipyretic and anti-inflammatory of Lonicerae japonicae[J]. Pharmacol Clin Chin Mater Med, 2012, 28: 115-117 (in Chinese).

    [3]Chinese Pharmacopoeia Committee. Pharmacopoeia of the People’s Republic of China[S]. PartⅠ. Beijing: China Medical Science Press, 2015 (in Chinese).

    [4]Guan HJ, Zhang X, Tu FJ, et al. Chemical constituents of Dendrobium officinale Kimuraet Migo[J]. Chin Tradit Herb Drugs, 2009, 40: 1873-1876 (in Chinese).

    [5]Wang DJ. Study on Chemical Constituents and Anti-H5Subtype AIV Activity of Leaves from Lonicera japonica Thunb. [D]. Taian: Shandong Agricultural University, 2013 (in Chinese).

    [6]Alqasoumi SI, Kader MSA. Constituents of the aerial parts of Lonicera etrusca growing in Saudi Arabia[J]. Nat Prod Sci, 2009, 15: 121-124.

    [7]Yao S, Xu NY, Chu XJ, et al. Chemical constituents of Rabdosia japonica var. glaucocalyx and their anti-complementary activity[J]. China J Chin Mater Med, 2013, 38: 199-203 (in Chinese).

    [8]Wang GS, Zhou XP, Yuan SJ, et al. Chemical constituents in the fruits of Lonicera macckii[J]. Chin J Med Chem, 2010, 20: 211-213 (in Chinese).

    [9]Kita M, Kigoshi H, Uemura D. Isolation and structure of korolkoside, a bis-iridoid glucoside from Lonicera korolkovii[J]. J Nat Prod, 2001, 64: 1090-1092.

    [10]Zhang C, Ma Y, Gao HM et al. Non-alkaloid constituents from Sophora flavescens[J]. China J Chin Mater Med, 2013, 38: 3520-3524 (in Chinese).

    [11]Zhu TF, Li P, Hu JJ, et al. A new secoiridoid glycoside from Picrorhiza scrophulariiflora[J]. Chin Tradit Herb Drugs, 2013, 44: 3260-3263 (in Chinese).

    [12]Li C, Dai Y, Zhang JB, et al. A new iridoid glycoside from Lonicerae Flos[J]. Chin Tradit Herb Drugs, 2013, 44: 2951-2954 (in Chinese).

    [13]Fan XN, Lin S, Zhu CG, et al. Aromatic chemicals and its active of Heteroplexis[J]. China J Chin Mater Med, 2011, 36: 48-56 (in Chinese).

    [14]Wang HL, Ren HC, Zou ZM. Study on antioxidative constituents in Rhizomes of Polygonum amplexicaule[J]. Chin Pharm J, 2011, 46: 819-822 (in Chinese).

    [15]Suan SL, Tang SA, Qin N, et al. Chemical constituents of Phymatopteris hastate and their antioxidant activity[J]. China J Chin Mater Med, 2012, 37: 1402-1407 (in Chinese).

    [16]Delnavazi MR, Hadjiakhoondi A, Delazar A, et al. Azerosides A and B: two new phloroacetophenone glycosides from the roots of Dorema glabrum Fisch. &CA Mey[J]. Med Chem Res, 2014, 24: 787-796.

    [17]Qin SJ, Li HJ, Li P, et al. Studies on chemical constituents of aerial parts of Lonicera dasystyla Rehd[J]. Chin Pharm J, 2008, 43: 662-664 (in Chinese).

    [18]Song YL, Wang HM, Ni FY, et al. Study on anti-inflammatory activities of phenolic acids from Lonicerae Japonicae Flos[J]. Chin Tradit Herb Drugs, 2015, 46: 490-495 (in Chinese).

This Article



Vol 51, No. 07, Pages 1110-1116

July 2016


Article Outline



  • Results and discussion
  • Experiment
  • References