Arginine succinyltransferase pathway deficiency in Pseudomonas donghuensis HYS affects its toxicity to Caenorhabditis elegans

QIN Ying-Qiu1 WU Ting-Ting1 GUI Zhe1 XIE Zhi-Xiong1

(1.College of Life Sciences, Wuhan University, Wuhan, Hubei, China 430072)

【Abstract】[Background] Pseudomonas donghuensis HYS is a high-siderophore-yielding strain isolated and identified by our laboratory from the Wuhan East Lake. HYS has strong toxicity to Caenorhabditis elegans. Previous studies have found that insertions in the argS gene in HYS can significantly reduce its toxicity to C. elegans. [Objective] To explore the function of argS gene and how it affects bacterial toxicity, and to provide a theoretical basis for further study on the mechanism of HYS toxicity. [Methods] The biological function of argS gene and the relationship between the arginine succinyltransferase (AST) pathway and bacterial toxicity were investigated with bioinformatics comparison, genetic analysis, physiological assay, and biochemical experiments. [Results] The argS gene in HYS encoded a protein that shared 88% similarity to the AST β subunit in P. aeruginosa. The deletion of argS prevented the HYS strain from using arginine as the sole carbon source for growth. Strains with the deletion of key genes in arginine decarboxylase (ADC) pathway, arginine dehydrogenase (ADH) pathway, and arginine deiminase (ADI) pathway could normally use arginine as the sole carbon source and had no significantly attenuated effect on the toxicity to C. elegans. Adding arginine to these strains significantly attenuated the toxicity and decreased their ability in producing siderophores. [Conclusion] AST pathway in P. donghuensis HYS can affect the toxicity to C. elegans by influencing the synthesis of the siderophores. This study lays the foundation for further studies on the mechanism of arginine metabolism and pathogenicity in Pseudomonas.

【Keywords】 Pseudomonas donghuensis HYS; argS gene; AST pathway; Toxicity;

【DOI】

【Funds】 National Natural Science Foundation of China (31570090) National Infrastructure of Natural Resources for Science and Technology Program of China (NIMR-2020-8)

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This Article

ISSN:0253-2654

CN: 11-1996/Q

Vol 47, No. 10, Pages 3257-3265

October 2020

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Article Outline

Abstract

  • 1 Materials and methods
  • 2 Results and analysis
  • 3 Discussion and conclusions
  • References