Microbial mechanism of sulfide inhibiting N2O reduction during denitrification in fluvo-aquic soil

SUI Wei-Kang1 LI Ji1 WU Xiao-Gang1 WU Qiao-Yu1 MA Yi-Ming1 ZHANG Xin-Yu1 ZHANG Xiao-Jun1

(1.State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China 200240)

【Abstract】[Background] The products of denitrification are N2O and N2. Thus it causes nitrogen loss and aggravates greenhouse effect. Sulfide has significant inhibitory effect on N2O reduction. However, the effect of sulfide on denitrifying bacteria and functional genes of denitrification is unclear. [Objective] This paper aims to explore the effect of sulfide on the accumulation of intermediate products of denitrification (NO and N2O), transcription of functional genes of denitrification (narG, nirS, nirK, and nosZ) as well as the microbial community structure in soil amended with or without carbon source. [Methods] Different levels of sodium sulfide (0 and 150 mg-S/kg-soil) combined different levels of glucose (0 and 1 000 mg-C/kg-soil) were set up in soil microcosms experiment. Robotized incubation system was used to monitor the amount of NO, N2O, and N2 accumulated during the incubation, and reverse transcription quantitative real-time PCR (RT-qPCR) was performed to quantify the transcriptions of functional genes of denitrification (narG, nirS, nirK, and nosZ). MiSeq platform based on high-throughput sequencing of 16S rRNA gene was used to analyze microbial community structure. [Results] Sodium sulfide significantly inhibited the reduction of N2O, but it had no significant effect on the accumulation of N2O. The addition of sodium sulfide significantly reduced the accumulated NO. Sodium sulfide significantly inhibited the activity of N2O reductase at the transcription level in a short time. Sodium sulfide inhibited the transcription of Azoarcus, Microvirga, Ensifer, Azohydromonas, Bacillus, Skermanella, Shinella, and Chthoniobacte. According to the query results of the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, sodium sulfide inhibited the growth of N2O-reducing denitrifying bacteria that cannot produce N2O. [Conclusion] The increase of soil sulfide caused by composting or other reasons leads to the inhibition of N2O reduction in denitrification process, which is due to the inhibition of nitrous oxide gene transcription and the selection impact of denitrifying bacteria. This study would be helpful to understand the microbial mechanism of the impact of sulfide on nitrogen metabolism.

【Keywords】 Sulfide; Denitrification; N2O reduction; Microbial community structure; Fluvo-aquic soil;

【DOI】

【Funds】 National Natural Science Foundation of China (31670105, 31971526, 31861133018)

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This Article

ISSN:0253-2654

CN: 11-1996/Q

Vol 47, No. 10, Pages 3114-3125

October 2020

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Article Outline

Abstract

  • 1 Materials and methods
  • 2 Results and analysis
  • 3 Discussion and conclusions
  • References