Bacterial community composition of the Antarctic moss Sanionia uncinata

XIAO Yao1 XIAO Yi-Lin1 WU Han1 CHAI Guang-Jun1 LI Zhi-Yong1

(1.State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China 200240)

【Abstract】[Background] Antarctic mosses harbor highly diverse polar microbes. However, the composition of Antarctic moss bacterial communities is not well understood, which limits the development and application of microbial resources in extreme habitats. [Objective] This study reveals the bacterial community of the Antarctic moss Saniania uncinata sampled randomly around the Antarctic Great Wall Station. [Methods] We used Illumina HiSeq high-throughput sequencing technology to analyse the 16S rRNA gene V4 region of the bacteria. [Results] A total of 273 367 bacterial sequences were obtained from the three moss samples. Totally, 9 759 operational taxonomic unit (OTUs) belonging to 14 phyla, 27 classes, and 50 genera, were grouped based on 97% sequence similarity. The dominant groups were Proteobacteria (30.70%), Bacteroidetes (19.67%), Verrucomicrobia (12.43%), Cyanobacteria (10.55%), and Actinobacteria (9.36%). Particularly, 56.73% of the bacteria in this moss were unclassified at the genus level. [Conclusion] The Antarctic moss Sanionia uncinata hosts phylogenetically diverse bacteria. This study provides a basis for the in-depth study of polar microbes.

【Keywords】 Antarctica; Sanionia uncinata; Bacterial diversity;

【DOI】

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    References

    [1] Campen R, Kowalski J, Lyons WB, et al. Microbial diversity of an Antarctic subglacial community and high-resolution replicate sampling inform hydrological connectivity in a polar desert [J]. Environmental Microbiology, 2019, 21 (7): 2290–2306

    [2] Coleine C, Stajich JE, Pombubpa N, et al. Altitude and fungal diversity influence the structure of Antarctic cryptoendolithic bacteria communities [J]. Environmental Microbiology Reports, 2019, 11 (5): 718–726

    [3] Lamilla C, Pavez M, Santos A, et al. Bioprospecting for extracellular enzymes from culturable Actinobacteria from the South Shetland Islands, Antarctica [J]. Polar Biology, 2017, 40 (3): 719–726

    [4] Cox F, Newsham KK, Robinson CH. Endemic and cosmopolitan fungal taxa exhibit differential abundances in total and active communities of Antarctic soils [J]. Environmental Microbiology, 2019, 21 (5): 1586–1596

    [5] Cerro-Gálvez E, Casal P, Lundin D, et al. Microbial responses to anthropogenic dissolved organic carbon in the Arctic and Antarctic coastal seawaters [J]. Environmental Microbiology, 2019, 21 (4): 1466–1481

    [6] Núñez-Montero K, Lamilla C, Abanto M, et al. Antarctic Streptomyces fildesensis So13. 3 strain as a promising source for antimicrobials discovery [J]. Scientific Reports, 2019, 9 (1): 7488

    [7] Bueno JL, Santos PAD, da Silva RR, et al. Biosurfactant production by yeasts from different types of soil of the South Shetland Islands (Maritime Antarctica) [J]. Journal of Applied Microbiology, 2019, 126 (5): 1402–1413

    [8] Vallesi A, Sjödin A, Petrelli D, et al. A new species of theγ-Proteobacterium Francisella, F. adeliensis sp. nov., endocytobiont in an antarctic marine ciliate and potential evolutionary forerunner of pathogenic species [J]. Microbial Ecology, 2019, 77 (3): 587–596

    [9] Lavian IL, Vishnevetsky S, Barness G, et al. Soil microbial community and bacterial functional diversity at Machu Picchu, King George Island, Antarctica [J]. Polar Biology, 2001, 24 (6): 411–416

    [10] Benavent-González A, Delgado-Baquerizo M, Fernández-Brun L, et al. Identity of plant, lichen and moss species connects with microbial abundance and soil functioning in maritime Antarctica [J]. Plant and Soil, 2018, 429 (1/2): 35–52

    [11] Roesch LFW, Fulthorpe RR, Pereira AB, et al. Soil bacterial community abundance and diversity in ice-free areas of Keller Peninsula, Antarctica [J]. Applied Soil Ecology, 2012, 61: 7–15

    [12] Cox F, Newsham KK, Bol R, et al. Not poles apart: Antarctic soil fungal communities show similarities to those of the distant Arctic [J]. Ecology Letters, 2016, 19 (5): 528–536

    [13] Chong CW, Silvaraj S, Supramaniam Y, et al. Effect of temperature on bacterial community in petroleum hydrocarbon-contaminated and uncontaminated Antarctic soil [J]. Polar Biology, 2018, 41 (9): 1763–1775

    [14] Niederberger TD, Sohm JA, Gunderson TE, et al. Microbial community composition of transiently wetted Antarctic Dry Valley soils [J]. Frontiers in Microbiology, 2015, 6: 9

    [15] Mangano S, Caruso C, Michaud L, et al. First evidence of quorum sensing activity in bacteria associated with Antarctic sponges [J]. Polar Biology, 2018, 41 (7): 1435–1445

    [16] Makhalanyane TP, van Goethem MW, Cowan DA. Microbial diversity and functional capacity in polar soils [J]. Current Opinion in Biotechnology, 2016, 38: 159–166

    [17] Bowman JS. Identification of microbial dark matter in Antarctic environments [J]. Frontiers in Microbiology, 2018, 9: 3165

    [18] Wei STS, Lacap-Bugler DC, Lau MCY, et al. Taxonomic and functional diversity of soil and hypolithic microbial communities in Miers Valley, Mc Murdo Dry Valleys, Antarctica [J]. Frontiers in Microbiology, 2016, 7: 1642

    [19] Newsham KK, Hopkins DW, Carvalhais LC, et al. Relationship between soil fungal diversity and temperature in the maritime Antarctic [J]. Nature Climate Change, 2016, 6 (2): 182–186

    [20] Castro-Sowinski S. The Ecological Role of Micro-Organisms in the Antarctic Environment[M]. Cham: Springer, 2019

    [21] Zhang T, Zhang YQ, Liu HY, et al. Diversity and cold adaptation of culturable endophytic fungi from bryophytes in the Fildes Region, King George Island, maritime Antarctica [J]. FEMS Microbiology Letters, 2013, 341 (1): 52–61

    [22] Holland-Moritz H, Stuart J, Lewis LR, et al. Novel bacterial lineages associated with boreal moss species [J]. Environmental Microbiology, 2018, 20 (7): 2625–2638

    [23] Tojo M, van West P, Hoshino T, et al. Pythium polare, a new heterothallic oomycete causing brown discolouration of Sanionia uncinata in the Arctic and Antarctic [J]. Fungal Biology, 2012, 116 (7): 756–768

    [24] Yamazaki Y, Tojo M, Hoshino T, et al. Characterization of Trichoderma polysporum from Spitsbergen, Svalbard archipelago, Norway, with species identity, pathogenicity to moss, and polygalacturonase activity [J]. Fungal Ecology, 2011, 4 (1): 15–21

    [25] Park M, Lee H, Hong SG, et al. Endophytic bacterial diversity of an Antarctic moss, Sanionia uncinata [J]. Antarctic Science, 2013, 25 (1): 51–54

    [26] Hedenäs L. Global phylogeography in Sanionia uncinata (amblystegiaceae: Bryophyta) [J]. Botanical Journal of the Linnean Society, 2011, 168 (1): 19–42

    [27] Bolger AM, Lohse M, Usadel B. Trimmomatic: a flexible trimmer for Illumina sequence data [J]. Bioinformatics, 2014, 30 (15): 2114–2120

    [28] Tang JY, Ma J, Li XD, et al. Illumina sequencing-based community analysis of bacteria associated with different bryophytes collected from Tibet, China [J]. BMCMicrobiology, 2016, 16 (1): 276

    [29] Martinez-Alonso E, Pena-Perez S, Serrano S, et al. Taxonomic and functional characterization of a microbial community from a volcanic englacial ecosystem in Deception Island, Antarctica [J]. Scientific Reports, 2019, 9 (1): 12158

    [30] Li W, Morgan-Kiss RM. Influence of environmental drivers and potential interactions on the distribution of microbial communities from three permanently stratified Antarctic lakes [J]. Frontiers in Microbiology, 2019, 10: 1067

    [31] Lee KC, Caruso T, Archer SDJ, et al. Stochastic and deterministic effects of a moisture gradient on soil microbial communities in the Mc Murdo Dry Valleys of Antarctica [J]. Frontiers in Microbiology, 2018, 9: 2619

    [32] Park CH, Kim KM, Kim OS, et al. Bacterial communities in Antarctic lichens [J]. Antarctic Science, 2016, 28 (6): 455–461

This Article

ISSN:0253-2654

CN: 11-1996/Q

Vol 47, No. 10, Pages 3083-3090

October 2020

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Article Outline

Abstract

  • 1 Materials and methods
  • 2 Results and analysis
  • 3 Discussion and conclusion
  • References