Assessment of the evolutionary history of Lauraceae in Xishuangbanna National Nature Reserve using DNA barcoding

HOU Qinxi1,2 CI Xiuqin1,2 LIU Zhifang1,2 XU Wumei3 LI Jie1

(1.Laboratory of Plant Phylogenetics and Conservation, Center for Integrative Conservation, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming 650223)
(2.University of Chinese Academy of Sciences, Beijing 100049)
(3.School of Energy and Environment Science, Yunnan Normal University, Kunming 650500)

【Abstract】Global biodiversity is diminishing at an unprecedented rate due to anthropogenic changes in the environment and establishing nature reserve is one of the most effective strategies for reducing biodiversity loss. Xishuangbanna, located in Southwest China, is a famous biodiversity hotspot and Lauraceae plants play an important role in the composition of its forest vegetation. To assess the role of Xishuangbanna National Nature Reserve (XNNR, established in 1958) in the conservation of evolutionary history of Lauraceae and to demonstrate the importance of combining phylogenetic information with biodiversity conservation, the evolutionary distinctiveness (ED), phylogenetic diversity (PD), species richness (SR), and endangerment categories of Lauraceae plants in Xishuangbanna were investigated. The results show that XNNR conserved only half of Lauraceae species (54.5%) found in Xishuangbanna, while 88.8% of PD was protected. However, there were still some areas (e.g. Daluo Town and Yiwu Town) with high PD that were not listed as conservation areas. A total of 19 species with high ED values (> 0.1) were found in Xishuangbanna, of which five species (26.3%) were not conserved in the XNNR, while 20 (37.0%) of 54 endangered species were not distributed in the nature reserve. Only three species with both high ED and endangerment categories were not found in the nature reserve. Our study shows that the XNNR protected a large proportion of PD and species with high conservation value, however, some important evolutionary history and endangered species of Lauraceae were still not conserved in the XNNR, indicating that the traditional assessment solely based on species richness could not incorporate phylogenetic information completely. We therefore conclude that PD should be considered in establishing nature reserves to maximize the evolutionary potential in an uncertain future.

【Keywords】 evolutionary history; phylogenetic diversity; evolutionary distinctiveness; nature reserve; DNA barcoding; Lauraceae;

【DOI】

【Funds】 National Natural Science Foundation of China (31770569, 31500454) Biodiversity Conservation Strategy of Strategic Biological Resources Service Network Plan by Chinese Academy of Sciences (ZSSD-013)

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    Footnote

    [1]. (1)Cited from the report of subpeoject “Local Plant Inventory and Protection in Southwest—Yunnan–Guizhou–Chongqing region (KFJ–1W–NO1–11)” of Native Plant Full Cover Protection (Pilot) Program (KFJ–1W–NO1–1) in China Botanical Garden Alliance Construction Project (KFJ–1W–NO1).

    References

    Abellán P, Sánchez-Fernández D, Picazo F, Millán A, Lobo JM, Ribera I (2013) Preserving the evolutionary history of freshwater biota in Iberian National Parks. Biological Conservation, 162, 116–126.

    Chen HF, Yi ZF, Schmidt-Vogt D, Ahrends A, Beckschäfer P, Kleinn C, Ranjitkar S, Xu JC (2016) Pushing the limits: The pattern and dynamics of rubber monoculture expansion in Xishuangbanna, SW China. PLoS ONE, 11, e0150062.

    Chen S, Yao H, Han JP, Liu C, Song JY, Shi LC, Zhu YJ, Ma XY, Gao T, Pang XH, Luo K, Li Y, Li XW, Jia XC, Lin YL, Leon C (2010) Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species. PLoS ONE, 5, e8613.

    Ci XQ, Li J (2017) Phylogenetic diversity and its application in floristics and biodiversity conservation. Biodiversity Science, 25, 175–181 (in Chinese with English abstract).

    De Vos JM, Joppa LN, Gittleman JL, Stephens PR, Pimm SL (2015) Estimating the normal background rate of species extinction. Conservation Biology, 29, 452–462.

    Faith DP (1992) Conservation evaluation and phylogenetic diversity. Biological Conservation, 61, 1–10.

    Forest F, Grenyer R, Rouget M, Davies TJ, Cowling RM, Faith DP, Balmford A, Manning JC, Proches S, Bank M, Reeves G, Hedderson TAJ, Savolainen V (2007) Preserving the evolutionary potential of floras in biodiversity hotspots. Nature, 445, 757–760.

    Gao LM, Liu J, Cai J, Yang JB, Zhang T, Li DZ (2012) Asynopsis of technical notes on the standards for plant DNAbarcoding. Plant Diversity and Resources, 34, 592–606 (in Chinese with English abstract).

    Ge XJ (2015) Application of DNA barcoding in phylofloristics study. Biodiversity Science, 23, 295–296 (in Chinese).

    Isaac NJB, Turvey ST, Collen B, Waterman C, Baillie JEM©生物多样性B (2007) Mammals on the EDGE: Conservation priorities based on threat and phylogeny. PLoS ONE, 2, e296.

    IUCN (2017) The IUCN Red List of Threatened Species. Version 2017-3. http: //www.iucnredlist.org/. (accessed on 2017-12-05)

    Kembel SW, Cowan PD, Helmus MR, Cornwell WK, Morlon H, Ackerly DD, Blomberg SP, Webb CO (2010) Picante: Rtools for integrating phylogenies and ecology. Bioinformatics, 26, 1463–1464.

    Klein C, Wilson K, Watts M, Stein J, Berry S, Carwardine J, Smith MS, Mackey B, Possingham H (2009) Incorporating ecological and evolutionary processes into continental-scale conservation planning. Ecological Applications, 19, 206–217.

    Kress WJ, Erickson DL, Jones FA, Swenson NG, Perez R, Sanjur O, Berminghan E (2009) Plant DNA barcodes and a community phylogeny of a tropical forest dynamics plot in Panama. Proceedings of the National Academy of Sciences, USA, 106, 18621–18626.

    Laity T, Laffan SW, González-Orozco CE, Faith DP, Rosauer DF, Byrne M, Miller JT, Crayn D, Costion C, Moritz CC, Newport K (2015) Phylodiversity to inform conservation policy: An Australian example. Science of The Total Environment, 534, 131–143.

    Larkin MA, Blackshields G, Brown NP, Chenna R, Mc Getti-gan PA, Mc William H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) Clustal W and Clustal X version 2. 0. Bioinformatics, 23, 2947–2948.

    Li HM, Aide TM, Ma YX, Liu WJ, Cao M (2007) Demand for rubber is causing the loss of high diversity rain forest in SWChina. Plant Conservation and Biodiversity, 16, 1731–1745.

    Li HW, Li J, Huang PH, Wei FN, Cui HB, van der Werff H (2008) Lauraceae. In: Flora of China, Vol. 7 (eds Wu ZY, Raven PH, Hong DY), pp. 102–254. Science Press, Beijing and Missouri Botanical Garden Press, St. Louis.

    Liu ZF, Ci XQ, Li L, Li HW, Conran JG, Li J (2017) DNAbarcoding evaluation and implications for phylogenetic relationships in Lauraceae from China. PLoS ONE, 12, e0175788.

    Lyubetsky V, Piel WH, Quandt D (2014) Current advances in molecular phylogenetics. Bio Med Research International, 2014, 596746.

    Mace GM, Norris K, Fitter AH (2012) Biodiversity and ecosystem services: A multilayered relationship. Trends in Ecology & Evolution, 27, 19–26.

    Miller MA, Pfeiffer W, Schwartz T (2010) Creating the CIPRES science gateway for inference of large phylogenetic trees. Gateway Computing Environments Workshop, 14, 1–8.

    Mishler BD, Knerr N, González-Orozco CE, Thornhill AH, Laffan SW, Miller JT (2014) Phylogenetic measures of biodiversity and neo- and paleo-endemism in Australian Aca-o diversity Science cia. Nature Communications, 5, 4473.

    Mooers AØ, Atkins RA (2003) Indonesia’s threatened birds: Over 500 million years of evolutionary heritage at risk. Animal Conservation, 6, 183–188.

    Moritz C (2002) Strategies to protect biological diversity and the evolutionary processes that sustain it. Systematic Biology, 51, 238–254.

    Oliver TH, Heard MS, Isaac NJB, Roy DB, Procter D, Eigenbrod F, Freckleton R, Hector A, Orme CD, Petchey OL, Proença V, Raffaelli D, Suttle KB, Mace GM, Martín-López B, Woodcock BA, Bullock JM (2015) Biodiversity and resilience of ecosystem functions. Trends in Ecology & Evolution, 30, 673–684.

    Pollock LJ, Rosauer DF, Thornhill AH, Kujala H, Crisp MD, Miller JT, Mc Carthy MA (2015) Phylogenetic diversity meets conservation policy: Small areas are key to preserving eucalypt lineages. Philosophical Transactions of the Royal Society B: Biological Sciences, 370, 20140007.

    Porebski S, Bailey LG, Baum BR (1997) Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Molecular Biology Reporter, 15, 8–15.

    Qian H, Jin Y, Ricklefs RE (2017) Phylogenetic diversity anomaly in angiosperms between eastern Asia and eastern North America. Proceedings of the National Academy of Sciences, USA, 114, 11452–11457.

    Redding DW, Mooers AØ (2006) Incorporating evolutionary measures into conservation prioritization. Conservation Biology, 20, 1670–1678.

    R Development Core Team (2013) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. http: //www. R-project. org. (accessed on 2017-11-30)

    Rodrigues ASL, Brooks TM, Gaston KJ (2005) Integrating phylogenetic diversity in the selection of priority areas for conservation: Does it make a difference?In: Phylogeny and Conservation (eds Purvis A, Gittleman JL, Brooks T), pp. 101–119. Cambridge University Press, London.

    Rodrigues ASL, Gaston KJ (2002) Maximising phylogenetic diversity in the selection of networks of conservation areas. Biological Conservation, 105, 103–111.

    Rolland J, Cadotte MW, Davies J, Devictor V, Lavergne S, Mouquet N, Pavoine S, Rodrigues A, Thuiller W, Turcati L, Winter M, Zupan L, Jabot F, Morlon H (2012) Using phylogenies in conservation: New perspectives. Biology Letters, 8, 692–694.

    Soutullo A, Dodsworth S, Heard SB, Mooers AØ (2005) Distribution and correlates of carnivore phylogenetic diversity across the Americas. Animal Conservation, 8, 249–258.

    Stamatakis A (2006) RAx ML-VI-HPC: Maximum likelihoodbased phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics, 22, 2688–2690.

    Wang S, Xie Y (2004) China Species Red List, Vol. 1: Red List. Higher Education Press, Beijing. (in Chinese)

    Webb CO, Ackerly DD, Kembel SW (2008) Phylocom: Software for the analysis of phylogenetic community structure and trait evolution. Bioinformatics, 24, 2098–2100.

    Winter M, Devictor V, Schweiger O (2013) Phylogenetic diversity and nature conservation: Where are we? Trends in Ecology & Evolution, 28, 199–204.

    Xishuangbanna Nature Reserve Comprehensive Investigation Group (1987) Xishuangbanna National Nature Reserve Comprehensive Investigation Reports. Yunnan Science and Technology Press, Kunming (in Chinese).

    Xishuangbanna National Nature Reserve Management Bureau, Yunnan Institute of Forest Inventory and Planning (2005) Xishuangbanna National Nature Reserve. Yunnan Education Publishing House, Kunming (in Chinese).

    Yek SH, Willliams SE, Burwell CJ, Robson SKA, Crozier RH (2009) Ground dwelling ants as surrogates for establishing conservation priorities in the Australian wet tropics. Journal of Insect Science, 9, 12.

    Zhu H, Wang H, Li BG, Zhou SS, Zhang JH (2015) Studies on the forest vegetation of Xishuangbanna. Plant Science Journal, 33, 641–726 (in Chinese with English abstract).

    Zhu H, Yan LC (2012) Native seed plants in Xishuangbanna of Yunnan. Science Press, Beijing (in Chinese).

This Article

ISSN:1005-0094

CN:11-3247/Q

Vol 26, No. 03, Pages 217-228

March 2018

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Article Outline

Abstract

  • 1 Methods
  • 2 Results
  • 3 Discussion
  • Supplementary Material
  • Footnote

    References