Genetic relationships of buckwheat species based on the sequence analysis of ITS and ndhF-rpl32
(2.Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China 030006)
(3.Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081)
(4.Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China 100081)
(5.China Office of Biodiversity International, Beijing 100081)
【Abstract】Buckwheat was originated in China and evolved into abundant species and genetic diversity. In order to effectively use germplasm resources of buckwheat and its wild relatives, the genetic relationships of buckwheat species were analyzed on 71 Fagopyrum samples including 10 species(subspecies, varieties and complex), which were collected mainly from Sichuan, Guizhou and Gansu provinces. Through the analysis of ITS and ndhF-rpl32 sequences and the construction of phylogenetic trees, the inter- and intra-specific relationships of these species were discussed. Results showed that the sequence length of ITS was 725 bp in the matrix, while the parsimony informative sites was 150, accounting for 20.7% of the total length. In the matrix of ndhF-rpl32 sequence, the length was 940 bp, while the parsimony informative sites was 158, accounting for 16.8% of total length. On one hand, the phylogenetic trees based on both ITS and ndhF-rpl32 sequencing data clearly separated the big-achene-group buckwheat (F. tataricum, F. cymosum complex, F. esculentum and F. esculentum ssp. ancestralis) from the small-achene-group buckwheat (F. gracilipes var. odontopterum, F. leptopodum var. grossii, F. leptopodum, F. densovillosum, F. gracilipes, and F. urophyllum). On the other hand, the phylogenetic tree based on ndhF-rpl32 sequences can be used to distinguish F. esculentum ssp. ancestralis and F. esculentum, which indicated that the ndhF-rpl32 sequence was more effective in analyzing the phylogenetic relationships of buckwheat species. These results provide a scientific basis for studies of taxonomy and establishing barcodes of Fagopyrum species.
【Keywords】 buckwheat; genetic relationships; ITS; ndhF-rpl32;
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