Co-expression of lignocellulase from termite and their endosymbionts

DU Jiao 1 JIANG Shuzhe 1 WEI Jianhua 1 SHEN Yulong 1 NI Jinfeng1

(1.State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong, China 266200)

【Abstract】Natural lignocellulosic materials contain cellulose, hemicellulose, and lignin. Cellulose hydrolysis to glucose requires a series of lignocellulases. Recently, the research on the synergistic effect of lignocellulases has become a new research focus. Here, four lignocellulase genes encoding β-glucosidase, endo-1,4-β-glucanase, xylanase and laccase from termite and their endosymbionts were cloned into pETDuet-1 and pRSFDuet-1 and expressed in Escherichia coli. After SDS-PAGE analysis, the corresponding protein bands consistent with the theoretical values were observed and all the proteins showed enzyme activities. We used phosphoric acid swollen cellulose (PASC) as substrate to measure the synergistic effect of crude extracts of co-expressing enzymes and the mixture of single enzyme. The co-expressed enzymes increased the degradation efficiency of PASC by 44% compared with the single enzyme mixture; while the degradation rate increased by 34% and 20%, respectively when using filter paper and corn cob pretreated with phosphoric acid as substrates. The degradation efficiency of the co-expressed enzymes was higher than the total efficiency of the single enzyme mixture.

【Keywords】 termite; lignocellulase; co-expression; synergism;

【Funds】 National Basic Research and Development Program of China (973 Program) (No. 2011CB707402) National Natural Science Foundation of China (Nos. 31272370, 30870085)

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(Translated by CHEN T)

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This Article

ISSN:1000-3061

CN: 11-1998/Q

Vol 35, No. 02, Pages 244-253

February 2019

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Article Outline

Abstract

  • 1 Materials and methods
  • 2 Results and analysis
  • 3 Discussion
  • References