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【摘要】目的 利用分子印迹技术制备左旋延胡索乙素(L-THP)分子印迹聚合物(MIPs),并进行固相萃取应用研究。方法 以L-THP为模板分子,甲基丙烯酸(MAA)为功能单体,乙二醇二甲基丙烯酸酯(EDMA)为交联剂,制备左旋延胡索乙素分子印迹聚合物(L-THP-MIPs),并进行固相萃取实验优化萃取条件,考察L-THP-MIPs吸附的选择性和专一性。结果 L-THP-MIPs能特异性地吸附L-THP,而对L-THP的结构类似物延胡索甲素未表现出明显的吸附行为。结论L-THP-MIPs对L-THP具有良好的选择性和专一性。

【关键词】 分子印迹技术;分子印迹聚合物;左旋延胡索乙素;固相萃取;甲基丙烯酸;乙二醇二甲基丙烯酸酯;延胡索甲素;


【基金资助】 广东省医学科学技术研究基金项目(A2015470); 广东食品药品职业学院自然科学研究项目(2014YZ010);

Preparation of molecular imprinted polymers for solid-phase extraction of L-tetrahydropalmatine

【Abstract】Objective To prepare the molecularly imprinted polymer (MIPs) of L-tetrahydropalmatine (L-THP) by the molecular imprinting technique and study on solid-phase extraction. Methods Using L-THP as template, methyl acrylic acid (MAA) as functional monmer, and ethylene glycol dimethacrylate (EDMA) as a cross-linking agent to prepare the L-THP-MIPs. A test was conducted to investigate the selectivity and the specificity of solid-phase extraction. Results The experiment showed that the MIPs had the specific adsorption to L-THP, but did not have the specific adsorption to corydaline the structural analogue with L-THP. Conclusion The L-THP-MIPs have a good selectivity and the specificity of L-THP.

【Keywords】 molecular imprinting technique; molecularly imprinted polymer; L-tetrahydropalmatine; solid-phase extraction; methyl acrylic acid; ethylene glycol dimethacrylate; corydaline;


【Funds】 Research Fund of Medical Science and Technology of Guangdong Province, China (A2015470); Natural Science Foundation of Guangdong Food and Drug Vocational College (2014YZ010);

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This Article


CN: 12-1108/R

Vol 47, No. 01, Pages 72-75

January 2016


Article Outline


  • 1 Instruments and materials
  • 2 Method
  • 3 Results and discussion
  • 4 Discussion
  • References