Aberration Characterization and Correction in Super-Resolution Localization Microscopy

Zhao Zeyu1,2 Zhang Zhaoning1,2 Huang Zhenli1,2

(1.Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, Hubei, China 430074)
(2.Key Laboratory for Biomedical Photonics, Department of Biomedical Engineering, Huazhong University of Science and Technology, Wuhan, Hubei, China 430074)

【Abstract】Super-resolution localization microscopy can achieve the ultra-high spatial resolution up to several nanometers by the single molecule localization and reconstruction from thousands or even tens of thousands of single molecule image, which provides unprecedented opportunities for studying the cell structures and biological phenomenon. However, the aberration (originating from the imperfection of the optical system or the inhomogeneity of the sample itself) distorts the raw images from single molecules, which decreases the final spatial resolution and even results in wrong results. The effects of several representative aberrations on super-resolution localization imaging are quantitatively characterized, and an aberration correction method based on the sample image itself is proposed. Simulation and experimental results show that the aberrations cause distortion of the point spread function and the decrease of the spatial resolution. The image quality can be restored by using the proposed aberration correction method.

【Keywords】 microscopy; fluorescence microscopy; super-resolution imaging; aberration correction; resolution; localization precision;

【DOI】

【Funds】 National Natural Science Foundation of China (91332103, 81427801)

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This Article

ISSN:0253-2239

CN: 31-1252/O4

Vol 37, No. 03, Pages 43-50

March 2017

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Article Outline

Abstract

  • 1 Introduction
  • 2 Influence of aberration on super-resolution localization microscopy
  • 3 Aberration Correction of Super-resolution Localization Microscopy
  • 4 Conclusion
  • References