Structure-Guided Development of Reversibly Photoswitchable Green Fluorescent Proteins Probe

Wang Sheng1,2,3 Chen Xuanze1,2,3 Chang Lei1,2,3 Xue Ruiying1,2,3 Sun Yujie1,2,3

(1.State Key Laboratory of Membrane Biology, Peking University, Beijing, China 100871)
(2.Biodynamic Optical Imaging Center, Peking University, Beijing, China 100871)
(3.School of Life Sciences, Peking University, Beijing, China 100871)

【Abstract】In recent years, people pay more and more attentions to the development of reversibly photoswitchable fluorescent proteins (RSFPs), because those fluorescent proteins can greatly promote the development and application of living-cell super-resolution imaging techniques. Reversibly photoswitchable fluorescent protein can be repeatedly and reversibly modulated by light with different wavelengths, thus it can be widely applied in many fields such as optical storage of high density data, the measurement of photochromic fluorescence resonance energy transfer, and super-resolution imaging based on the principle of reversible saturable optical linear fluorescence transitions (RESOLFT). The recent development of green RSFPs is reviewed from the key amino acids involved in RSFPs design. We also make a brief discussion of the relationship between structure and optical characteristics of RSFPs, serving as the reference for further structure-guided development of better RSFPs.

【Keywords】 microscopy; optical microscopy; reversibly photoswitchable; fluorescent protein; super-resolution imaging; fluorescent probe; green fluorescent protein;

【DOI】

【Funds】 National Natural Science Foundation of China (21573013, 21390412, 31271423, 31327901) National High-tech Research and Development Program of China (863 Program) (SS2015AA020406)

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(Translated by caizhijian)

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This Article

ISSN:0253-2239

CN: 31-1252/O4

Vol 37, No. 03, Pages 22-31

March 2017

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Article Outline

Abstract

  • 1 Introduction
  • 2 Introduction of fluorescent protein (FP)
  • 3 Reversibly photoswitchable FP developed based on the jellyfish-sourced FPs
  • 4 The reversibly photoswitchable FP developed from the coral-sourced fluorescent proteins
  • 5 Conclusions
  • References