降钙素原胶体金试纸条显色分析仪

王霞1 陈飞1 宿宸铭1 黎先龙2

(1.北京理工大学光电学院光电成像技术与系统教育部重点实验室, 北京 100081)
(2.北京中科同和信息技术有限公司, 北京 100088)

【摘要】为了实现降钙素原浓度的定量测量, 设计了基于树莓派的CMOS图像传感器胶体金试纸条显色分析仪, 对胶体金试纸条拍照获取试纸显色反应的图像颜色特征量。首先, 根据降钙素原胶体金试纸条的显色特性对CMOS传感器进行合理的白平衡校正, 使CMOS传感器具有较好的色彩还原性。然后, 为解决不同设备间颜色管理的差异, 通过对NCS标准色卡拍照, 利用多项式回归法建立了图像传感器RGB空间到XYZ空间的模型, 并对仪器进行了颜色特性化校正。最后, 针对试纸显色不均匀的问题, 经过图像处理后, 选择显色区域内具有代表性的像素点建立图像颜色特征量与样本浓度值的数学模型, 实现了待测样本浓度的精确测量。实验结果表明, 该胶体金试纸条显色分析仪的精度可达93.8%, 重复测量误差小于1.5%, 有效测量范围为0.1~ 35ng/mL。该分析仪成本低、测量速度快, 具有一定的应用价值。

【关键词】 色度分析; 胶体金; 颜色空间; 多项式回归; 降钙素; 浓度测量;

【DOI】

【基金资助】 博士点基金资助项目 (No.20131101130002)

Download this article

    References

    [1]WANG SH, ZHENG D ZH, FANG SH CH.Detecting method of quantitative colloidal gold test strip concentration[J].Journal of Beijing University of Aeronautics and Astronautics, 2009, 35 (8) :942-945. (in Chinese)

    [2]WANG Y, BAI Y J, ZHANG Y CH, et al..Design and reasearch of a photoelectrical apparatus for laterflow based on CCD[J].Chinese Journal of Sensors and Actuators, 2008, 21 (8) :1370-1373. (in Chinese)

    [3]KIM S, PARK J.Development of a test strip reader for a lateral flow membrane-based immunochromatographic assay[J].Biotechnology and Bioprocess Engineering, 2004, 9 (2) :127-131.

    [4]HONG G W.LUO M R, RHODES P A.A study of digital camera colorimetric characterization based on polynomial modeling[J].Color Res.Appl., 2001, 26 (1) :76-84.

    [5]WANG Y.Design of a Colloidal-gold Strip reader with High Accuracy[D].Hangzhou:Zhejiang University, 2013. (in Chinese)

    [6]XU Y F, LIU W Y, ZUO K L, et al..Reasearch on hierarchical color correction method[J].Opt.Precision Eng., 2004, 12 (1) :15-20. (in Chinese)

    [7]ZHAO Z Q, WANG R G, ZHENG X F, et al..Measurement of chroma of LED display with color CCD camera[J].Opt.Precision Eng., 2013, 21 (3) :575-582. (in Chinese)

    [8]ZHAO P, WANG W J, CHEN W.Research on hierarchical colour correction method[J].Engineering and Applications, 2015, 51 (6) :158-162. (in Chinese)

    [9]QIU X B, LIU CH CH, MAUK M G.A portable analyzer for pouch-actuated, immunoassay cassettes[J].Sensors and Actuators B:Chemical, 2011, 160 (1) :1529-1535.

    [10]BARNARD, FUNT B.Camera characterization for color research[J].Color Res.Appl., 2002, 27 (3) :152-163.

    [11]CHEUNG T L V, WESTLAND S.Color camera characterisation using artificial neural networks[C].IS&T/SID Tenth Color Imaging Conference, 2002:117-120.

    [12]SUN H C, TIAN R, DING N N, et al..Automatic white balance algorithm based histogram equalization and its FPGA implementation[J].Chinese Journal of Scientific Instrument, 2015, 36 (12) :222-226. (in Chinese)

    [13]LI B, XU D, XIONG W H, et al..Color constancy using achromatic surface[J].Color Res.Appl., 2010, 35 (4) :304-312.

    [14]KAWAKAMI R, ZHAO H X, TAN R T, et al..Camera spectral sensitivity and white balance estimation from sky images[J].International Journal of Computer Vision, 2013, 105 (3) :187-204.

    [15]XU M F, WANG L Q, YUAN B.Auto white balance algorithm of high definition electronic endoscope[J].Infrared and Laser Engineering, 2014, 43 (9) :3110-3115. (in Chinese)

    [16]YAO H T, TIAN Q Q, SU W, et al..Reasearch on automatic white balance algorithm based on color histogram equalization[J].Journal of Changchun University of Science and Technology:Natural Science Edition, 2014, 37 (4) :104-107. (in Chinese)

This Article

ISSN:1004-924X

CN: 22-1198/TH

Vol 25, No. 03, Pages 562-568

March 2017

Downloads:0

Share
Article Outline

摘要

  • 1 引言
  • 2 胶体金显色分析仪的构成及原理
  • 3 胶体金显色分析仪的关键算法
  • 4 测量实验与结果
  • 5 结论
  • 参考文献