Supervisor(s): Central Station of Chinese Medicinal Materials Information Sponsor(s): Central Station of Chinese Medicinal Materials Information; State Food and Drug Administration CN：44-1286/R
Journal of Chinese Medicinal Materials is supervised by Central Station of Chinese Medicinal Materials Information and sponsored by Central Station of Chinese Medicinal Materials Information and State Food and Drug Administration. The journal covers research article of Chinese herbal medicine planting and raising technology, resource exploitation and utilization, concocted processing maintenance of medicinal herbs, identification, ingredient, pharmacology, preparations, and pharmacy.
The journal is included in CA, JST and CSCD.
Objective: To study the effect of reactive oxygen species on female-male flower ratio of Schisandra chinensis in order to improve the yield. Methods: Spraying different concentration solution of H2O2, paraquat and sodium dithionite on the leaves of Schisandra chinensis before fruit bud initiation. Results: Exogenous sodium dithionite (O2– carrier) increased the female-male flower ratio greatly from 7.6% to 42.3%. H2O2 and paraquat (OH–carrier) had little effect. Conclusion: The regulation effects vary according to different ROS. Exogenous sodium dithionite has the best effect.
Objective: The impacts of lead (Pb) stress on the leaf photosynthetic pigment and the growth of Rabdosia rubescens was studied, in order to provide a basis for planting area selection and growth regulation. Methods: Taking chlorophyll a, chlorophyll b, carotenoids, growth rate, biomass and Pb content as the indexes, the Ramets hydroponic experiments at Pb concentration levels (135, 270 and 540 mg/L) in the time (20, 35 and 50 d) were carried out. Photosynthetic pigment content was determined by spectrophotometer, and Pb mass fraction was detected with plasma emission spectrometer. Results: There was uncertain effect on chlorophyll and carotenoid synthesis in different Pb concentrations in early period (20 and 35 d). At the time of 50 d, the chlorophyll content was higher in the low–mid Pb concentrations, significantly lower in the high Pb concentration compared with the control group, and there were no significant differences on carotenoid contents in different Pb concentrations. Conclusion: Low–mid Pb concentrations can promote chlorophyll synthesis, and the bioaccumulation of high Pb concentration can inhibit the chlorophyll synthesis, and then restrict the growth of Rabdosia rubescens.
Objective: To find out the high yield cultivation measures for Arctii Fructus. Methods: Completely randomized block experiment design method was used in the field planting, to analyze the effect of different cultivation way on agronomic characters, phenological phase, quality and quantity of Arctii Fructus. Results: Arctium lappa planted on August 28 had the best results of plant height, thousand seeds weight and yield. The highest yield of Arctii Fructus was got at the density of 1 482 plants/667 m2. Arctiin content was in an increase trend with the planting time delay and planting density increasing. The plant height, thousand seeds weight, yield and arctiin content by split application of fertilizer were significantly higher than that by one-time fertilization. Compared with open field Arctium lappa, plant height, yield, arctiin content and relative water content of plastic film mulching Arctium lappa was higher by 7.74%, 10.87%, 6.38% and 24.20%, respectively. In the topping Arctium lappa, the yield was increased by 11.09%, with 39.89% less branching number. Early planting time and topping shortened the growth cycle of Arctium lappa plant. Conclusion: The high-yield cultivation measures of Arctii Fructus are: around August 28 to sowing, planting density of 1 482 plants/667 m2,split application of fertilizer for four times, covering film on surface of the soil and topping in bolting.
Objective: To detect concentrations of serum gonadal hormones(testosterone, estradiol and progesterone) in musk-secreting period and estrus of Moschus berezovskii, and to study the association of serum gonadal hormones concentrations and musksecreting. Methods: The concentrations of serum gonadal hormones were detected with magnetic particle separation ELISA. Results: During musk-secreting period, concentration changes of three serum gonadal hormones showed clear regularity, three crests occurred synchronously. Before musk-secreting period, testosterone, estradiol and progesterone concentrations were at its lower level, in prime musksecreting period, they increased rapidly to respective highest peak; at later musk-secreting period, they quickly dropped to close to its previous levels before musk-secreting period. During estrus, serum testosterone concentration increased to lower peaks than that at later musk-secreting period. Estradiol remained at a low level and progesterone level was closed to zero. Serum testosterone concentrations in prime musk-secreting period were 114. 4–190. 5 times of estrus. During musk-secreting period, there were significant positive correlation among three serum gonadal hormone levels, a positive correlation between musk yield and serum testosterone levels, and negative correlation of musk yield with serum estradiol and progesterone levels as well as musk deer ages. Conclusion: Serum testosterone concentrations in prime musk-secreting period increase to the highest levels, which can provide reference in musk secretion induced by artificial means.
Objective: To establish a sensitive and specific high performance liquid chromatography (HPLC) method for simultaneous determination of alizarin anthraquinones (1-methoxy-2-hydroxy anthraquinone, 1, 2-dimethoxy-3-hydroxy anthraquinone, rubiadin-1-methylether, 1, 3-dihydroxy-2-methoxy anthraquinone, rubiadin) in Morinda officinalis from different habitats. Methods: The analysis was carried out on an Ecosil C18 column (250 mm × 4.6 mm, 5 μm) and detected with an UV detector at the wavelength of 277 nm. Gradient elution was carried out with acetonitrile-0.2% phosphoric acid at the flow rate of 0.8 mL/min. The column temperature was set at 30 °C. Results: The calibration curves were linear in the range of 0.2856–34.27 μg/mL for 1-methoxy-2-hydroxy anthraquinone (r = 0.9999), 0.3268–39. 22 μg/mL for 1, 2-dimethoxy-3-hydroxy anthraquinone (r = 0.9999), 0.3450–41.40 μg/mL for rubiadin-1-methylether (r = 0.9999), 0.1248–14.98 μg/mL for 1, 3-dihydroxy-2-methoxy anthraquinone (r = 0.9999) and 0.0508–6.096 μg/mL for rubiadin (r = 0.9994), respectively. The average recoveries of the five components were 99.4%, 100.2%, 101.4%, 97.2%, 103.2%, respectively. The content of active components of samples from 10 different hatitats were in the range of 0.0025–0.0722 mg/g, 0.0016–0.0658 mg/g, 0.0022–0.0684 mg/g, 0.0182–0.3965 mg/g and 0.0014–0.0179 mg/g. Conclusion: The established method is accurate, reliable, and can be used for the simultaneous determination of the five components in Morinda officinalis, which provides a scientific basis for the quality evaluation of Morinda officinalis.
Objective: To study the material composition of Tibetan medicine “Brag-zhun”, and to provide the basis for interpretation of its source and reference for the establishment of quality standards. Methods: Pharmacognosy routine method was used in macroscopic and microscopic identifications, inductively coupled plasma spectrometry was carried out to determine 26 kinds of mineral elements, and X-ray diffraction analysis was used for the crystalline phase detection of Brag-zhun. The content of calcium, organic matter, humic acid and fulvic acid was determined according to GB/T 14610-2008, “technical specification for soil analysis”, GB/T 11957-2001 and capacity titration, respectively. Moisture, ash content, acid insoluble ash content and water soluble extract were determined with reference to the Chinese Pharmacopoeia( 2010 edition). Results: Animal feces were commonly found in Brag-zhun, the main crystal phase was SiO2, 26 kinds of mineral elements content in total was about 3%, 13 batches of medicinal materials in organic matter content was 29.03%–71.79%. Humic acid content was 28.37%–58.51%; fulvic acid content was 19.69%–41.43%; moisture was 5.2%–11.7%; total ash content was 17.82%–64.39%; acid insoluble ash content was 2.10%–39.09%; water soluble extract was 28.39%–57.40%. Conclusion: The traditional record of Brag-zhun from molten juice is lack of scientific basis. The mineral elements in Brag-zhun exist in amorphous. Brag-zhun contains a lot of organic matter including humic acid and fulvic acid, and inorganic matter mainly comes from sediment. The organic matter in rock has gone through a long geological evolution.
Objective: To study the chemical constituents of Phyllanthus emblica. Methods: The chemical constituents were isolated and purified by silica gel, polyamide and Sephadex LH-20 chromatography. Their structures were elucidated by physicochemical properties and spectral analysis. Results: 13 compounds were isolated and identified as Triacontanol (1), Triacontanoic acid (2), β-Amyrin ketone (3), Betulonic acid (4), Daucosterol (5), Lupeol acetate (6), β-Amyrin-3-palmitate (7), Gallic acid (8), Betulinic acid (9), Ursolic acid (10), Oleanolic acid (11), Quercetin (12) and Rutin (13). Conclusion: Compounds 1, 2, 4, 6, 7, 9, 10 and 11 are obtained from Phyllanthus emblica for the first time.
Objective: To study the chemical constituents of fermented Antrodia camphorata powder. Methods: 15 compounds were isolated from Antrodia camphorata by Silica gel column chromatography, ODS column chromatography, gel column chromatography, preparative liquid phase chromatography separation technique, as well as recrystallization. Results: On the basis of their physical and chemical properties and spectral data, their structures were identified as Ferulic acid (1), Inositol (2), β-Sitosterol (3), Vanillin (4), Vanillic acid (5), Butyric acid (6), Daucosterol (7), p-Hydroxycinnamic acid (8), Lauric acid (9), Inosine (10), Uridine (11), Adenine (12), D (+)-Sucrose (13), Arachidic acid (14) and Guanosine (15). Conclusion: Compounds 1, 5, 6 and 8–15 are isolated from fermented powder for the first time.
Objective: To study the chemical constituents of Cremastra appendiculata. Methods: The compounds were isolated by repeated column chromatography with silica gel, Sephadex LH-20 and ODS-HPLC. The structures were elucidated by analysis of spectroscopic data (1H-NMR and 13C-NMR). Results: Ten compounds were isolated from the EtOAc extract in the tuber of Cremastra appendiculata. Their structures were identified as shancigusin Ⅰ (Ⅰ), 4-O-β-D-glucopyranosyl cinnamate (Ⅱ), bulbocodin D (Ⅲ), blestriarene A (Ⅳ), 7-hydroxy-2, 4-dimethoxy phenanthrene (Ⅴ), coelonin (Ⅵ), militarine (Ⅶ), gastrodine (Ⅷ), 3-hydroxyphenylpropionic acid (Ⅸ) and cinnamic acid (Ⅹ). Conclusion: Compounds Ⅰ–Ⅲ, Ⅸ and Ⅹ are isolated from Cremastra appendiculata for the first time.
Objective: To investigate the chemical constituents of peanut hull. Methods: Several chromatography methods such as silica gel and Sephadex LH-20 combined with recrystallization were applied to isolate the compounds. Based on spectrum technologies (MS, 1H-NMR and 13C-NMR) and physico-chemical methods,structures of isolated compounds were identified. Results: Twelve compounds were isolated and elucidated as luteolin (1), diosmetin (2), 5, 7, 3', 4'-tetrahydroxy-8-prenylflavone (3), 5, 7, 3'-trihydroxy-4'-methoxy-8-prenylflavone (4), eriodicrtyol (5), racemoflavone (6), hydnocarpin (7), 5, 7-dihydroxy chromone (8), 5-hydroxy-chromone-7-O-β-D-glucoside (9), ferulic acid(10), β-sitosterol (11) and daucosterol ( 12). Conclusion: Except compounds 1, 5 and 8, all compounds are obtained from peanut hull for the first time.
