Sponsored by Tianjin Institute of Pharmaceutical Research；Chinese Pharmaceutical Association
ISSN 0253-2670 CN 12-1108/R
24 issues per year
Discipline(s): Medical Science
Current Issue: Issue 02, 2019
Chinese Traditional and Herbal Drugs is supervised by Chinese Pharmaceutical Association and sponsored by Tianjin Institute of Pharmaceutical Research and Chinese Pharmaceutical Association. Launched in 1970, the journal is an academic journal with a broad scope in publishing research papers, brief reports, reviews, dissertations, and special treatises on the recent achievements of basic study, production, quality control, and clinic application on traditional Chinese medicine and Chinese materia medica. The journal is included in CA, JST and CSCD.
Vol 50,No. 02
Objective For the purpose of finding new bioactive compounds from natural resources, the phytochemical investigation on the fruiting bodies of Amauroderma rude was carried out. Methods The chemical constituents from A. rude were isolated by various technologies, such as silica gel, Sephadex LH-20, MCI-gel resin, and high performance liquid chromatography. The isolated compounds were elucidated by spectroscopic methods, including extensive 1D, 2D NMR, and ESI-MS techniques. Results Three compounds (1–3) including a new norlignan, were isolated from this mushroom. Their structures were identified as 4-ethyoyl- E-3-(3,4-dihydroxybenzylidene)-5-(3,4-dihydroxyphenyl)furan-2-one ( 1), esculetin ( 2), and caffeic acid ( 3) . Conclusion Compound 1 is a new compound isolated from this fungus, which is identified as amauroderin A.
Preparation, spectroscopy and molecular modelling studies of inclusion complex of vincamine with hydroxypropyl-β-cyclodextrin
Vol 50,No. 02
Objective The inclusion complex of vincamine (VIN) and hydroxypropyl-β-cyclodextrin (HP-β-CD) was prepared and characterized. Molecular simulation method was used to study the formation mechanism of inclusion complex. Methods The inclusion complex of VIN/HP-β-CD was prepared by saturated solution. The preparation technology of VIN/HP-β-CD inclusion complex was optimized by orthogonal design, and taking the drug loading of the inclusion complex as the index. The stability constant of inclusion complex between VIN and HP-β-CD was studied by UV–Vis spectrometry titration, and the inclusion ratio was determined by Job plots method. The VIN/HP-β-CD inclusion complex was characterized by scanning electron microscope (SEM), X-ray powder diffractometry (XRD), infrared spectroscopy (IR), thermal analysis techniques (TG and DSC), and nuclear magnetic resonance (1H, 2D NMR). The water solubility of the VIN/HP-β-CD inclusion complex was measured and the stability test was conducted in the simulated human gastric juice and intestinal fluid environment. Molecular docking and molecular dynamics were used to study the forming mechanism of supramolecular system of VIN/HP-β-CD. Results Using saturated solution method, the optimum conditions of inclusion were 1:1 for molar ratio of VIN and HP-β-CD, 40 ℃ for inclusion temperature, 7 h for inclusion time and volume ratio of methanol to water (1:6) as solvent; Job curve and UV–vis spectroscopy showed that inclusion ratio of host-guest inclusion complexes was 1:1; After VIN formed inclusion complexes with HP-β-CD, its solubility increased from 0.04 mg/mL to 16.5 mg/mL, and the thermal decomposition temperature of VIN increased from 240.5 ℃ to 306.1 ℃. 1H-NMR and NOESY spectra indicated that the inclusion complex was formed by the a-ring of VIN entering from the large end of HP-β-CD. Quantum calculation and molecular docking indicated that the optimal inclusion mode was consistent with the results of NMR studies. Molecular dynamics studies showed that VIN can penetrate into the hydrophobic cavity of HP-β-CD in water environment, and the interaction between host and guest was strengthened. The space size of host–guest matched better. Conclusion The solubility and thermal stability were significantly improved after the formation of inclusion complex with VIN and HP-β-CD. Hydrophobicity, hydrogen bonding, and van der Waals forces were the main driving forces for inclusion complex formation.
Mechanisms of Gexia Zhuyu Decoction on anti-angiogenesis of hepatic fibrosis based on regulation of VEGF mRNA expression mediated by HIF-1α
Vol 50,No. 02
Objective To explore the mechanism of improving angiogenesis of hepatic fibrosis by Gexia Zhuyu Decoction (GZD) through the regulation of the mRNA expression of VEGF mediated by HIF-1α. Methods A total of 108 Wistar rats were randomly divided into normal group ( n = 18), model group ( n = 18), N-acetylcysteine (NAC) group ( n = 18), high-dose GZD group (GD, n = 18), middle-dose GZD group (GZ, n = 18), and low-dose GZD group (GX, n = 18). Hepatic fibrosis model was established by intraperitoneal injection of 50% CCl 4–olive oil solution (1 mL/kg) twice a week for nine weeks. Each group was administered while model established, until the rats were sacrificed. Normal group and model group were ig given sterile water 10 mL/(kg·d), NAC group was ig given NAC 0.1 g/(kg·d), GD, GZ, GX groups were given 26, 7.8, and 3.9 g/(kg·d) GZD by oral gavage. At 3, 6, and 9 weeks, rats in the corresponding groups were randomly sacrificed. Masson staining was used to make pathological specimens, immunohistochemical analysis of Col-Ⅳ and laminin was also performed, and real-time PCR was used to detect the mRNA expression of HIF-1α and VEGF. Western blotting was used to detect the protein expression levels of VEGF and VEGFR2. Results Compared with model group, NAC group and GD group significantly inhibited the expression of LN in the extracellular matrix at 9 weeks ( P < 0.05). Both NAC and GD groups significantly inhibited the expression of extracellular matrix Col-Ⅳ, especially at 6 weeks and 9 weeks. NAC group, GD group, and GZ group can significantly inhibit the high expression of HIF-1α in liver tissue of rats with liver fibrosis ( P < 0.05). At 6 weeks and 9 weeks of administration, NAC and GD groups significantly inhibited the high expression of VEGF mRNA in liver tissue ( P < 0.05). Both GZD and NAC could inhibit the protein expression of VEGF and VEGFR2 in liver tissue. Conclusion GZD can regulate the expression of VEGF mRNA mediated by HIF-1α, which may be one of the key mechanisms of its anti-angiogenesis for hepatic fibrosis.
Lipidomics study of protective effect of Radix Salviae Miltiorrhizae and Rhizoma Ligustici Chuanxiong on rats with focal cerebral ischemia injury based on UPLC-Q/TOF-MS
Vol 50,No. 02
Objective To study the regulatory effect of freeze-dried substances of Radix Salviae Miltiorrhizae and Rhizoma Ligustici Chuanxiong Decoction on lipid metabolism abnormality in focal cerebral ischemia rats. Methods The focal cerebral ischemia rat model was established with the monofilament method. The rats were randomly divided into normal group, sham operation group, model group, and drug treatment group. Plasma was collected after the last dosage and UPLC-Q/TOF-MS was used to study the plasma lipidomics. The lipidomics data were subjected to orthogonal partial least square discriminant analysis (OPLS-DA), and the lipid metabolite changes were examined before and after the intervention of Radix Salviae Miltiorrhizae and Rhizoma Ligustici Chuanxiong. Results The focal cerebral ischemia rat model was successfully replicated. The freeze-dried substances of Radix Salviae Miltiorrhizae and Rhizoma Ligustici Chuanxiong Decoction obviously reversed the abnormal lipid metabolism profile in the focal cerebral ischemia rat model. The plasma lipid biomarkers of ischemia injury rat were PS (21:0/0:0), PG (12:0/17:0), C16 sphinganine, phytosphingosine, PE [18:1(9Z)/0:0], PC (16:1/2:0), PC (0:0/18:0), PC (16:1/0:0), PC (16:0/0:0), PC (18:2/0:0), PC (18:1/0:0), PC (18:0/0:0), and PC (20:5/0:0), respectively. Conclusion Freeze-dried substances of Radix Salviae Miltiorrhizae and Rhizoma Ligustici Chuanxiong Decoction have protective effect on cerebral ischemia injury, which may be related to the regulation of abnormal lipid metabolism, especially for phosphatidylcholines (PCs).
Exploration and application of a new method for rapid extraction of DNA from Chinese medicinal materials
Vol 50,No. 02
Objective To build an equipment-free nucleic acid extraction dipstick methodology that can obtain amplification-ready DNA from Chinese medicinal materials in 30 s. Methods A new rapid DNA extraction method which was suitable for traditional Chinese medicine, with equipment-free nucleic acid extraction dipstick methodology that can obtain amplification-ready DNA from Chinese medicinal materials in 30 s was verified. The filter paper strip or disk for the adsorbed nucleic acid could be directly amplified by the reaction system. Results This method could extract the nucleic acid successfully. The extracted DNA was amplified, and the results were consistent with the traditional extraction methods. Conclusion The new rapid DNA extraction method was simple, fast, and low cost, which would make nucleic acid extraction become more and more popular, and no longer confined to professional and laboratory environment. This study provides a new idea for the application and development of molecular pharmacology.
Vol 50,No. 02
Objective Taking the concentration process of liquorice extract as the research object, we constructed the dynamic simulation process for concentration of Chinese medicinal materials by combining experimental analysis with theoretical simulation, which has provided the model support and theoretical basis for process study and equipment development of concentration of Chinese medicinal materials. Methods The corresponding relationship between boiling point and saturated vapor pressure of liquorice solution was determined by dynamic method. The experimental data were fitted by thermodynamic model to obtain relevant parameters. On this basis, the simulation process of liquorice water extract concentration was constructed by using ASPEN PLUS. According to the simulation of dynamic process, the effects of heating power, feed rate, and vacuum degree on liquorice solution concentration in an external thermal concentrator were discussed. Finally, the equations about concentration time and heating power were obtained by simulation. Results The results of parameter fitting were A ij = 1.63, A ji = 2.32, B ij = 336.38, B ji = 792.00, and C ij = 0.5. Finally, the functional equation for the concentration time and heating power was t = 2 329 c1H/ c0Q. Conclusion In this study, the effects of different process parameters on the concentration of Chinese medicinal materials were analyzed by simulation and related theories, and a simple prediction of the concentration process was realized. It also perfected and optimized the process simulation data, filled the relevant scientific research gap, and was of great significance to industrial guidance. Firstly, the relevant experimental data were obtained by fitting the thermodynamic model with the relevant experimental data. Under the ideal process conditions, the influencing factors of liquorice concentration process were analyzed and discussed by dynamic simulation. It was concluded that heating power was the key factor affecting the concentration process, and the concentration time was gradually decreased with the increase of heating power. However, their functional relationship was non-linear. At the same time, the functional equation could be used to roughly predict the concentration time of Chinese medicinal materials. To a certain extent, this fills in the gap between the related theoretical research and data of chemical thermodynamics, which provides theoretical support for the process research and equipment development of the concentration process of Chinese medicinal materials.