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Brief

Microbiology China is supervised by Chinese Academy of Sciences, sponsored by Chinese Society for Microbiology and Institute of Microbiology, CAS. Impact factor is 1.336.
Editorial Board

Editor-in-Chief: Hao Rongqiao

Bacterial community composition of the Antarctic moss Sanionia uncinata

XIAO Yao;XIAO Yi-Lin;WU Han;CHAI Guang-Jun;LI Zhi-Yong

Microbiology China,2020,Vol 47,No. 10

Arginine succinyltransferase pathway deficiency in Pseudomonas donghuensis HYS affects its toxicity to Caenorhabditis elegans

QIN Ying-Qiu;WU Ting-Ting;GUI Zhe;XIE Zhi-Xiong

Microbiology China,2020,Vol 47,No. 10

Microbial mechanism of sulfide inhibiting N 2O reduction during denitrification in fluvo-aquic soil

SUI Wei-Kang;LI Ji;WU Xiao-Gang;WU Qiao-Yu;MA Yi-Ming;ZHANG Xin-Yu;ZHANG Xiao-Jun

Microbiology China,2020,Vol 47,No. 10

[Background] The products of denitrification are N 2O and N 2. Thus it causes nitrogen loss and aggravates greenhouse effect. Sulfide has significant inhibitory effect on N 2O reduction. However, the effect of sulfide on denitrifying bacteria and functional genes of denitrification is unclear. [Objective] This paper aims to explore the effect of sulfide on the accumulation of intermediate products of denitrification (NO and N 2O), transcription of functional genes of denitrification ( narG, nirS, nirK, and nosZ) as well as the microbial community structure in soil amended with or without carbon source. [Methods] Different levels of sodium sulfide (0 and 150 mg-S/kg-soil) combined different levels of glucose (0 and 1 000 mg-C/kg-soil) were set up in soil microcosms experiment. Robotized incubation system was used to monitor the amount of NO, N 2O, and N 2 accumulated during the incubation, and reverse transcription quantitative real-time PCR (RT-qPCR) was performed to quantify the transcriptions of functional genes of denitrification ( narG, nirS, nirK, and nosZ). MiSeq platform based on high-throughput sequencing of 16S rRNA gene was used to analyze microbial community structure. [Results] Sodium sulfide significantly inhibited the reduction of N 2O, but it had no significant effect on the accumulation of N 2O. The addition of sodium sulfide significantly reduced the accumulated NO. Sodium sulfide significantly inhibited the activity of N 2O reductase at the transcription level in a short time. Sodium sulfide inhibited the transcription of Azoarcus, Microvirga, Ensifer, Azohydromonas, Bacillus, Skermanella, Shinella, and Chthoniobacte. According to the query results of the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, sodium sulfide inhibited the growth of N 2O-reducing denitrifying bacteria that cannot produce N 2O. [Conclusion] The increase of soil sulfide caused by composting or other reasons leads to the inhibition of N 2O reduction in denitrification process, which is due to the inhibition of nitrous oxide gene transcription and the selection impact of denitrifying bacteria. This study would be helpful to understand the microbial mechanism of the impact of sulfide on nitrogen metabolism.

Symbiotic microorganism diversity in comb and feces of fungus-growing termite

ZHANG Shuo;LIN Zi-Jia;XIAO Yuan-Xi;LI Jing-Jing;LI Zhi;NI Jin-Feng

Microbiology China,2020,Vol 47,No. 10