Sponsored by World Federation of Chinese Medicine Societies
ISSN 1673-7202 CN 11-5529/R
12 issues per year
Discipline(s): Medical Science
Current Issue: Issue 02, 2019
《World Chinese Medicine》is a comprehensive academic journal of Chinese medicine. The magazine is aimed at domestic and foreign medical workers, especially the medical staff of TCM medical, teaching, scientific research and management, and is the first in China to be global. Publicly issued Chinese medicine academic journals.
Study on the Fingerprint of Xanthium sibiricum L. from Different Origins and the Content Determination of Its Main Components
Vol 14,No. 02
Objective: To study the HPLC fingerprint of Fructus Xanthii, and to evaluate the quality of Fructus Xanthii from different habitats and batches, and to provide a reference for the formulation of the overall quality standard of Xanthium herbs. Methods: A total of 10 batches of Fructus Xanthii were determined by high performance liquid chromatography, and the data were analyzed by the similarity calculation software of the National Pharmacopoeia Committee of Chinese Pharmacopoeia (CTP), and the common pattern of the fingerprint of Fructus Xanthii was established. Results: A total of 16 fingerprint peaks were identified, and a common fingerprint pattern of Fructus Xanthii was established. The contents of four main components in Xanthium sibiricum from different habitats were determined. Conclusion: The method is stable and reproducible, and the established fingerprint can be used for quality evaluation and quality control of Fructus Xanthii.
Vol 14,No. 02
With the rapid development of bionic technology in 21st Century, the “odor” can be objectively quantified, which breaks the limitation of traditional experiential identification and is widely used in the quality control of traditional Chinese medicine because of its integrity. In this paper, the concept, principles and characteristics of electronic nose technology are introduced in detail, and its application status and the existing problems in the field of Chinese medicine are summarized systematically in recent years. With the progress of electronic nose technology, it will provide a new opportunity for the objective evaluation of traditional Chinese medicine and in-depth study of correlation between exterior character and internal quality on traditional Chinese medicine.
A comparative Study on the Aesculus hippocastanum L. and Chinese Buckeye Seed Based on Chinese and English Literature Databases
Vol 14,No. 02
Aesculus hippocastanum L. (horse chestnut seed) belongs to the family of Aesculus L. species, which are the dry mature seeds for horse chestnut. The seeds, bark and leaves of horse chestnut have been used for hundreds of years as a traditional herbal medicine, which can be applied to treat diseases such as the fever and hemorrhoids. Modern studies have shown that the seed has been the most widely used due to the most active ingredient. Chinese buckeye seeds are recorded in Chinese Pharmacopoeia, which are dry and mature seeds of Aesculus chinensis Bge., Aesculus chinensis Bge. var. chekiangensis (Hu et Fang) Fang or Aesculus wilsonii Rehd. Chinese buckeye seeds, warm in property, sweet in flavor, with the channel tropisms in liver and stomach, have the functions of soothing the liver to regulate its qi flow, regulating the stomach and relieving the pain, which can be used to treat the diseases such as liver and stomach qi stagnation, chest abdominal distension, and epigastric pain. In this paper, based on the Web of Science (WoS), PubMed, CNKI database, the chemical composition, pharmacological effects, clinical application and other aspects of Aesculus hippocastanum L. and Chinese buckeye seeds reported in the Chinese and English literature were compared and summarized. The results showed that Aesculus hippocastanum L. contained saponins, flavonoids, phenols, fatty acids and other chemical constituents had the functions of anti-inflammation, anti-oxidation, anti-allergy, anti-cancer, enabling it to be clinically applied to treat chronic venous insufficiency, heart blood vessels and other diseases. The chemical compositions of Chinese buckeye seed were almost the same as those of Aesculus hippocastanum L., and it had pharmacological effects such as anti-inflammation and gastrointestinal protection, enabling it to be used clinically for treating cerebral hemorrhage, cerebral thrombosis and trauma. The research results showed that the Aesculus hippocastanum L. and Chinese buckeye seeds had different pharmacological actions and clinical applications. It is speculated that the possible reason might be that the research focus of the two was different, or there existed a certain difference in some chemical compositions between them, which needs to be further studied. This paper will lay the foundation for the introduction of Aesculus hippocastanum L. in China, as well as the study of its medicinal value and its development.
Qualitative Study on Chemical Composition of Ginger Magnolia with Different Methods Based on UHPLC-UV-Q-TOF-MS/MS
Vol 14,No. 02
A qualitative analytical method based on quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was developed for identification of multi-constituents of raw Magnolia (RM) and processed Magnolia (GM). UHPLCQ-TOFMS/MS qualitative analysis was performed under positive and negative ion modes and a total of 35 chemical compounds were identified. The analysis data were subjected to a principle component analysis with a t-test. Five peaks were found to be the main difference ( P < 0.05) between RM and GM. The results indicated that there was higher magnatriol, magnolol, tetradecanoic acid, palmitic acid, chlorogenic acid in GM than in RM. However, there were fewer levels of globulol in the GM than RM, which may be the main reason for different clinical efficacy of RM and GM.
Rapid Identification and Characterization of Chemical Components in the Active Part of Herba Cynomorii by UPLC/LTQ-Orbitrapa MS
Vol 14,No. 02
Objective: To analyze and identify the chemical constituents from the active part of Herba Cynomorii using ultra performance liquid chromatography/liner ion trap-orbitrapa mass spectrometry (UPLC/LTQ-Orbitrapa MS). Methods: The analysis was performed on an Acquity UPLC BEH C 18 reverse phase column (100 mm × 2.1 mm, 1.7 μm) under the following conditions: injection volume: 5 μL; flow rate: 0.3 mL/min; mobile phase: 0.1% formic acid water (A)–acetonitrile (B); gradient elution: 0–7 min, 10%–40% B; 7–21 min, 40% B; 21–21 min, 40%–80% B; 21–28 min, 80% B. MS conditions: Electrospray ionization (ESI) source; scanning mode: positive and negative ion modes. Results: After the mass spectra data were analyzed, a total of 20 compounds were identified, including nucleosides, phenolic acids, flavonoid glycosides, Coumarins and amino acid. Conclusion: By using UPLC/LTQ-Orbitrapa MS method, the main chemical constituents from Herba Cynomorii could be accurately identified.
Study on Mechanism of Liver Injury in Rats Induced by Emodin Based on Lable-free Proteomics Technology
Vol 14,No. 02
Objective: To investigate the influence of 4-week’s emodin on protein expression of rat’s liver tissue. Methods: SD rats were given emodin 100 times more than the clinical dose of Rhubarb for 4 weeks and were divided into four groups (HG1, HG2, HG3, HG4). Protein expression in liver tissue was detected by Lable-free proteomic technology. Results: By screening, there were 158, 650, 219, and 378 differential proteins in HG1, HG2, HG3, and HG4 groups respectively, which were found to be mainly associated with cellular and metabolic processes by GO analysis. Among them, there were 25 differential proteins in the four groups, and GO classification indicated that 10 of the 25 proteins were proteins related to catalytic activity. α-2-HS-glycoprotein (thermostable glycoprotein) expression was upregulated by the presence of emodin, and the concentration of this protein positively correlated with the severity of liver disease; and cathepsin family egg-cysteine-rich protease expression was downregulated. Conclusion: Long-term administration of emodin increases the probability of liver injury, which may be due to the down-regulation of the expression of cysteine-containing proteases, which leads to the disorder of tissue function and leads to the appearance of liver injury, and this result has also been confirmed in the low-dose group.
Extraction and Separation of Bioactive Flavonoids from Oxytropis falcata by Polyamide Chromatography Combined with Medium-pressure Liquid Chromatography
Vol 14,No. 02
Objective: To extract and isolate the flavonoids from Oxytropis falcata (OF), and screen bioactive flavonoids on human lung adenocarcinoma A549 cells in vitro. Methods: Crude extraction was extracted from OF by ethyl acetate. Ethyl acetate extraction was further separated by polyamide column chromatography with gradient elution and purified by medium-pressure liquid chromatography to obtain the flavonoids; High performance liquid chromatography (HPLC), mass spectrometry (MS) and nuclear magnetic resonance spectroscopy (NMR) were used to analyze and elucidate the structure of the obtained flavonoids. CCK-8 analysis was used to detect the inhibited viability of flavonoids on human lung adenocarcinoma A549 cells in vitro. Results: There were two flavonoids isolated from OF ethyl acetate fraction, and were identified as 7-hydroxy dihydroflavone and 2',4'-dihydroxy dihydrochalcone. Purity of them were both greater than 98% by HPLC analyses; The results of CCK-8 showed that 7-hydroxy dihydroflavone and 2',4'-dihydroxy dihydrochalcone could significantly inhibit the viability of A549 cells in a dose-dependent manner. The IC 50 values were 137.6 μg/mL and 63.83 μg/mL at 48 h, respectively. Conclusion: The two flavonoids have effective bioactivities on inhibiting proliferation of human lung adenocarcinoma A549 cells, which could provide the experimental basis for the further studies on anti-lung cancer mechanism. Polyamide column chromatography is suitable for enrichment and isolation of flavonoids. Polyamide column chromatography combined with medium pressure liquid chromatography is simple and easy to prepare various flavonoids. Thus, the method could provide a reference for obtaining flavonoids from Chinese medicines.