Sponsor(s): Institute of Zoology, the Chinese Academy of Sciences; the Entomological Society of China
12 issues per year
Current Issue: Issue 12, 2019
The aims of the Acta Entomologica Sinica are to report the most recent achievements and advances in entomological sciences, and to promote academic exchanges among entomological researchers in China and abroad. The journal publishes original research papers, review articles and short communications concerning various aspects of entomology and various groups of insects and mites, and accepts submissions in Chinese or English. Currently, original research papers are organized in four regular subsections, i.e., Physiology and Biochemistry, Toxicology and Resistance, Ecology and Pest Management, and Evolution and Systematics, and irregular subsections as necessary, such as Pathology and Medical Insects, for each issue.
Gao Xiwu, Liu Shusheng, Wan Fanghao, Xia Qingyou, Xu Weihua, Zhao Xiaofan
Differential expression of multicopper oxidase 4 gene and biological effects after RNAi and feeding on different rice varieties in Sogatella furcifera (Hemiptera: Delphacidae)
Acta Entomologica Sinica,2019,Vol 62,No. 12
[Aim] The study aims to analyze the expression difference of multicopper oxidase 4 gene and the biological effects after RNAi and feeding on different rice varieties in Sogatella furcifera, so as to provide a theoretical basis for fundamental function research of this gene. [Methods] The nucleotide and amino acid sequences of MCO4 gene were analyzed with bioinformatic methods. The MCO4 gene in the 3rd instar nymphs of S. furcifera was silenced by RNAi; the silencing efficiencies of microinjection method and feeding method were compared, and the effect of RNAi of MCO4 gene on the survival rate of the 3rd instar nymphs was studied. After the feeding of S. furcifera on rice varieties with different insect resistance for 24 h, the changes in the expression level of MCO4 in S. furcifera adults were detected by RT-qPCR, and their body weight was detected. [Results] The full-length cDNA of MCO4 gene is 2 166 bp, encoding 721 amino acids. RNAi by microinjection method successfully silenced the MCO4 gene of S. furcifera, with much higher silencing efficiency than that by feeding method. The survival rate of the 3rd instar nymphs with silenced MCO4 was significantly lower than that of the normal nymphs. Compared with the adults fed on the susceptible cultivar TN1, the adults fed on the resistant variety IR26 had significantly higher expression level of MCO4 and significantly reduced body weight. [Conclusion] Microinjection method can more efficiently silence the MCO4 gene of S. furcifera. Silence of MCO4 gene has significant effects on the survival rate of S. furcifera, and we speculate that MCO4 plays an important role in feeding of S. furcifera on rice.
Acta Entomologica Sinica,2019,Vol 62,No. 12
[Aim] To screen out the most stably expressed reference genes in Lygus pratensis under specific conditions. [Methods] qRT-PCR was adopted to determine expression levels of 10 candidate reference genes including alpha-tubulin, beta-tubulin, actin, elongation factor, succinate dehydrogenase, ubiquitin, transcription initiation factor TFIID subunit, glutathione S-transferase, ribosomal protein L32, and TATA-box binding protein genes in six categories of samples of L. pratensis including adults of different genders, different adult tissues, different developmental stages, adults with different lambda-cyhalothrin resistance, and adults respectively exposed to different temperatures and different insecticides. BestKeeper, geNorm, NormFinder, and RefFinder were adopted to analyze the mRNA expression stability of the candidate reference genes. [Results] According to the results of different analysis methods based on the effective qRT-PCR data, it was concluded that the most stably expressed reference gene was RPL32 for different adult tissues and adults of L. pratensis with different lambda-cyhalothrin resistance. The most stably expressed reference genes for the groups of adults exposed to different temperatures, adults of different genders, adults exposed to different insecticides and different developmental stages were UBQ, SDHA, β-tubulin and TAF, respectively. Based on the comprehensive analysis from geNorm and RefFinder calculation, the best combinations of stably expressed reference genes for the groups of different adult tissues, adults of different genders, different developmental stages, adults with different lambda-cyhalothrin resistance, and adults exposed to different temperatures and different insecticides were RPL32 + TAF, SDHA + GST, TAF + GST + UBQ, RPL32 + GST, UBQ + ACT + β-tubulin, and β-tubulin + TAF + RPL32, respectively. [Conclusions] All the most stably expressed reference genes and their best combinations obtained from this study could be used as reference genes for the expressiion analysis of genes in L. pratensis for future research.