Sponsored by Chinese Society for Microbiology
ISSN 1000-8721 CN 11-1865/R
6 issues per year
Current Issue: Issue 04, 2019
Chinese Journal of Virology, an academic periodical established in 1985, publishes Original Articles, Brief Reports, Reviews, and so on, covering the advances and achievements of fundamental and applied research concerning human, animal, plant, and insect viruses as well as bacteriophages and prions. The subscribers of Chinese Journal of Virology are mainly workers in research institutes, universities, and other institutions of virological and biological studies in China, as well as world-known databases and libraries. Chinese Journal of Virology is supervised by the China Association for Science and Technology, sponsored by the Chinese Society for Microbiology, run by the National Institute for Viral Disease Control and Prevention, China CDC, and published by its Editorial Office. The International Standard Serial Number is ISSN 1000-8721; the Domestic Journal Number is CN 11-1865/R; the Domestic Postal Distribution Code is 82-227 (domestically distributed by all local post offices in China); the Overseas Distribution Code is BM6448 (distributed by China International Book Trading Corporation). Chinese Journal of Virology has been published bimonthly since 2005 and is now distributed worldwide. Chinese Journal of Virology is included in the Outstanding S&T Journals of China, Chinese Core Journals of Science and Technology, A Guide to the Core Journals of China (published by Peking University), Research Center for Chinese Science Evaluation, Wuhan University, and Chinese Science Citation Database source journals. In addition, it is indexed in CA, BA, CBST, MEDLINE (PubMed), and WPRIM. Chinese Journal of Virology has been included in the databases of CNKI (Disc Edition) since December 30, 2008. It began to tentatively offer Advance Online Publication in 2009 and has been formally offering Advance Online Publication since 2011. The composite impact factor of Chinese Journal of Virology is 1.355, according to the Annual Report for Chinese Academic Journal Impact Factors (Basic Medical Sciences) (2015, Volume 13), ranking top among the journals of basic medical and biological sciences. The homepage of Chinese Journal of Virology on CNKI is http://bdxb.cbpt.cnki.net. Chinese Journal of Virology started a pilot program of bilingual publication on November 8, 2016 to publish papers in both Chinese and English on CNKI net.
Honorary Editor-in-Chief: HOU Yunde
Editor-in-Chief: SHU Yuelong
LIU Xiufan, FANG Rongxiang, WU Guizhen, JIN Qi, LIANG Mifang, LI Mengfeng, XIA Ningshao, TAN Wenjie, XU Wenbo, WANG Yumei
Identification and Molecular Characterization of a Novel Virus of the Family Orthomyxoviridae (Longchuan Virus)
Vol 35,No. 04
The distribution and molecular characterization of arbovirus were performed in Guangdong Province, China, in 2013. A strain of virus was isolated from Culex pipiens fatigans in Longchuan County. The virus resulted in a cytopathogenic effect in C6/36 lines. To enable the identification and molecular characterization of the virus, viral particles were purified by density gradient centrifugation with cesium chloride. The viral shape was observed using the negative-staining methods under electron cryomicroscopy. RNA was amplified by single-primer isothermal amplification and deep sequencing was carried out on the Ion Torrent platform. The generated sequences were subjected to quality control and classified by BLAST. CLC Genomic Workbench was used to assemble the sequences. The representative sequences from the Orthomyxoviridae family were downloaded from GenBank. Phylogenetic trees were constructed using MEGA. The amino-acid and nucleotide sequences were compared among new and representative strains using ClustalW2. The transmission electron microscopy showed that these novel viral particles were spheres and were 100 nm in diameter. Six segments with complete open-reading frames were obtained by deep sequencing. The amino acid identification of 26.38%–67.23% among five segments (PB1, PB2, PA, NA and HA) was obtained with representatives from the Quaranjavirus genus of the Orthomyxoviridae family. PB1 and PB2 had the highest and lowest homology, respectively. A closer relation was not found with the other viruses. In summary, we identified a novel arbovirus within the Orthomyxoviridae family from Longchuan County. The virus was named the “Longchuan virus”. This is the first report of isolation of the Longchuan virus from Culex pipiens fatigans in China.
Inhibitory Effects of Tubeimoside 1 on the Proliferation and Genomic Replication of Herpes Simplex Virus Type 1 and Type 2
Vol 35,No. 04
Tubeimoside (TBMS) 1 is a unique pentacyclic triterpenoid saponin composed of bayogenin and four sugars: glucose, rhamnose, xylose and arabinose. Previous studies have shown that TBMS1 has activity against the human immunodeficiency virus. We investigated the inhibitory effects of TBMS1 on the proliferation and genomic replication of herpes simplex virus type 1 (HSV-1) and 2 (HSV-2). TBMS1 was extracted and isolated from the tubers of [ Bolbostemma paniculatum (Maxim) Franquet (Cucurbitaceae)], and its purity in our study was great than 95%. The toxicity of HSV-1 and HSV-2 was determined by the plaque assay. The antiviral activity of TBMS1 was determined by cytopathic effects and plaque-inhibition test, and real-time quantitative polymerase chain reaction (PCR) was used to detect the effects of TBMS1 on HSV genomic replication. TBMS1 showed significantly inhibitory effects on the proliferation of HSV-1/F and HSV-2/333 strains, and on copies of UL27, UL52 and UL54 in a dose-dependent manner. TBMS1 of high concentration (0.39 μg/mL–0.78 μg/mL) was not toxic and showed inhibitory effects on the proliferation of the ACV-resistant strain HSV-1/106. TBMS1 had obvious inhibitory effects on the proliferation and genomic replication of HSV-1 and HSV-2.
Association between Mutation of the E4 Gene of Human Papillomavirus Type 16 and the Development of Cervical Cancer
Vol 35,No. 04
Human papillomavirus type 16 (HPV16) is one of the most common types seen in cervical cancer (CC). A study has shown that the variation in the nucleic-acid sequence of the E4 gene may affect the carcinogenicity of HPV16 . In previous research, we found that the E2 gene of HPV16 was associated with CC. We wished to understand the mutation of the E4 gene of HPV16 and explore its correlation with CC development. Hence, we tested the variants of the E4 gene in the DNA sequences of 118 HPV16-positive CC patients and 151 HPV16-positive asymptomatic individuals. We constructed a phylogenetic tree, respectively, with the Neighbor-joining method. The distribution of HPV16 variants was significantly different between the case group and control group ( P = 0.015), the Asian–American variant of which was likely to relate to CC occurrence. The analysis of DNA sequences showed significant difference in the distribution of eight variants between the case group and control group ( P < 0.05). In the European variant, two mutations, C3384T (L18L) and A3449G (P39P), were associated with the initiation and development of CC. Our results suggest that the mutation of the E4 gene had certain correlation with CC development.
Analyses of Surveillance Results for Influenza B Virus and the Characteristics of Its Hemagglutinin Gene in Yunnan Province, China, 2017
Vol 35,No. 04
Between 2017 and 2018, the influenza virus was very prevalent in China. In particular, the influenza B virus (IBV) with the Yamagata (By) lineage and the H1N1 and H3N2 subtypes were prevalent in China. To evaluate the monitoring results for IBV and the molecular characteristics of the hemagglutinin (HA) gene of the IBV in Yunnan Province, China, 2017, a total of 21 672 specimens were collected from the Influenza Surveillance Hospital of Yunnan Province, China, in 2017 and processed using real-time reverse transcription polymerase chain reaction. The nucleic acid-positive specimens were isolated by MDCK cells using the HA agglutination test and inhibition test for IBV identification. Twenty four IBV strains were selected randomly for high-throughput sequencing. The characteristics of their HA genes were analyzed using MEGA software. In 2017, the data (monitoring and outbreak monitoring) of sentinel hospitals in Yunnan Province, China showed that IBV was one of the dominant strains for the influenza epidemic. Nine outbreaks (42.86%) caused by the Victoria (Bv) lineage and four outbreaks (19.05%) by the By lineage were detected. The HA gene of IBV showed no obvious variation. Strengthening monitoring, improving monitoring sensitivity, and providing timely warnings are crucial to lessen the risk of influenza epidemics.